[HARRISON & barlow] SLIME-PRODUCING ORGANISM 



103 



Potash Gelatin Plates. — Plate cultures were made with potash 

 gelatin containing various carbohydrates and incubated at 18 to 22° C. 

 In 24 hours no colonies were visible. In 2 days colonies were visible in 

 all the plates. Further observations are tabulated below. 



Medium. 



Days. 



All. 



KPS, KPGa. ! 2 



KPS, KPD, 

 KAG, KPL, 

 KPGa. 



KPS, KPD. 

 KS, KP, KPDn 



KS. 



KP. 

 KP. 



KP, KPGa. 



KPG, KPD, 

 KPI 



All except KPS 



KPS. 



3 

 3 



4 and 5 



4 

 2 to 10 



3, 4, 5 

 5 



5 



7 



10 



Colony, Pigment, Liquefaction, Crystals. 



Punctiform colonies. Under the microscope, round, 

 entire, homogeneous, finely granular, brownish. 



Colonies larger; surface ones up to 5 m.m., deep 

 ones smaller. Colonies darker than the pigmented 

 gelatin, a deep green-blue color in the gelatin. 



Green-blue tint in the gelatin where thickly seeded. 

 Pigment grains through the whole depth of the 

 gelatin, none in the colonies. No pigmentation in the 

 other plates. 



Rich in pigment grains from this time on. KPGa 

 showed fewest grains and evenly scattered. 



Colonies — surface raised, round, entire, brownish 

 by transmitted light, green-blue by reflected light. 



Colonies .5 to 1.5 m.m. in diameter. Surface colo- 

 nies raised, wet-shining, and coalescing. Deep and 

 surface colonies were slimy and could be drawn out 

 in threads. Colonies dark with numerous pigment 

 grains at the centre. 



Pigmentation green-blue but in parts with fewer 

 colonies a deep blue pigmentation. 



Slight pigmentation. 



In 2 days a yellow-green tint, in 5 days grains in 

 the centres of some colonies and a few in the 

 gelatin. 



Liquefaction began in 5 days. 



In 3 days no pigment, in 4 days emerald green. 

 In 5 days darker with green-blue and pale blue. 

 Liquefaction began in 3 days and gelatin was lique- 

 fied in 4 days. 



Gelatin light yellow-green, color gone in ô days. 



Crystals very numerous in the gelatin, the simple 

 ones as double pyramids with square bases placed 

 base to base. No crystals in the plates with car- 

 bohydrates. 



Liquefaction began. 



Liquefaction began. 



Liquefied. Liquefaction began where colonies were 

 most numerous, the discrete colonies floated in the 

 liquefied gelatin which later became turbid and 

 mucous-like and drew out in slimy threads. 



Liquefaction delayed beyond 10 days. 



