[HARRISON & barlow] SLIME-PRODUCING ORGANISM 105 



The crystals elsewhere described do not form m cultures Avith 

 pigment grains. 



Plate Cultures in Liehig Peptone Agar at 20° C. — In 5 days the 

 surface colonies averaged 1 m.m. in diameter, round, raised, wet-shining, 

 white. Under the microscope, round, entire, finely granular, brownish 

 by transmitted light, thinner and lighter at the margins, sometimes 

 one zoned. Surface colonies readily coalesced with a line at the union. 



In 5 days deep colonies were up to .5 m.m. in diameter, round, oval 

 or elliptical, yellowish and opaque. The growth could be drawn out in 

 slimy threads from all the colonies. There was a yellowish tint in the 

 colonies and medium but no pigment grains. Crystals were present. 

 The cultures had an unpleasant acid odor. 



Plate cultures at 25-2T° C. grew more rapidly, otherwise the same 

 as at 20° C. 



Agar Cultures. — Agar of the following, composition was made up : 

 Agar 1^, tap Avater 100 c.c, normal sodium hydrate 1 c.c. This was* 

 heated, filtered and divided into two portions, one was tubed and re- 

 ferred (to later as plain agar, and to the other was added 1^ of sucrose. 

 The reaction was alkaline — 5° to phenolphthalein. The tubes were 

 inclined, inoculated and incubated at 13 — 22° C. 



Growth was slow on plain agar. In 18 days it had increased some- 

 what and could be drawn out in slimy threads. There was no pigment 

 and Pehling's solution was not reduced. 



Growth was more rapid and abundant on the sucrose agar. The 

 water of condensation and the growth on the slant were very slimy, but 

 there was no pigment. Growth increased up to 18 days when there was 

 no pigment and Fehling's solution was strongly reduced. In 33 days 

 there was no pigment. 



Agar and Sucrose Agar at 20"^ C. — Xutrient agar of the following 

 composition, — Liebig's Extract 5^, peptone 1'/, salt, 5%, agar 1.2^, 

 acidity + 4.5° — was used alone or with various percentage of sucrose. 

 The inclined agar was inoculated and incubated at 18° — 22° C. 



Growth was rapid and abundant on these media, but was somewhat 

 retarded on that containing 20^ and 25^ of sucrose. Pigmentation was 

 unequal ; plain agar showed only a slight discoloration, that with sucrose 

 1/10;^ a faint blue pigmentation and with 25^ none. The 1^, 

 5^, 10;^ and 20<^ sucrose agars produced a deep blue pigmentation. All 

 with sucrose, 1^ or more, reduced Fehling's solution after 6 days. 



A similar lot of media was kept at 25 — 27° C. There was growth 

 on all the agars in 24 hours, and in three days there was abundant 

 growth, that on the 20^ sucrose agar was a little restrained. 



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