106 



ROYAL SOCIETY OF CANADA 



There was no pigment in 30 hours but in 3 days there was jjigmont 

 in all the agars vnth. sucrose. With 5^ and with 10;^ sucrose there 

 was a deep blue pigmentation in 3 days, and a faint blue pigmentation 

 in tubes containing 1/10^, 1^, and 20^ sucrose. In 4 days most oi 

 the pigment had disappeared and in 5 days from inoculation it had 

 completely discharged. 



Cultures on 1^, hfc, 10^ and 20% sucrose agar strongly reduced 

 Fehling's solution. 



Plain agar and 20^ sucrose agar cultures were alive at the end 

 of 55 days. 



Blood Serum. — On Loeffler's serum kept at 37° C. growth was 

 raised, shiny, and yellowish white in color. There was no liquefaction. 

 The culture was then placed at a lower temperature but no pigmentation 

 or liquefaction ensued. 



Beef Peptone Bouillon. — Bouillon vnÛ\ Liebig's Extract, .25^, 

 peptone 1^, salt .5^, was made neutral to phenolphthalein and from it 

 bouillons were prepared having the following alkalinites;-5°,-10°,-20°. 

 -35°,-40° and 60°. A little sucrose was added to some of the tubes, and 

 such media is marked S. 



Growth in Alkaline Bouillons at 18° — 22° C. 



Numerals denote de- 

 grees of alkalinity. 

 S stands for sucrose. 

 + neutral. 



-5S, -103, -20S. 

 + , —5", —10°. —20". 



— 40S, —40. 



—35". 



—60'^. 



+ S, — 5S, — lOS. 



--, -.5°, —10", -40°S. 



—20", —40". 



+ S, — 5S, -lOS. 



+ S, -5S, — lOS. 

 -40S. 



Growth, Slimimess, Pigment, Reduction. 



In 24 hours turbid and with ropy sediment, in- 

 creasing for 27 days, abundant growth. 



In 8 days no growth, in 12 days turbidity and ropy 

 sediment increasing for 27 days. 



In 20 days a little growth. 



In 27 days no growth. 



In 20 days so slimy as to draw out in threads. 



In 27 days so slimy as to draw out in threads. 



In 27 days not so slimy as to draw out in threads. 



In 20 days and in 27 days strong green-blue pig- 

 mentation from the surface 10 m.m. or less deep, in 

 the +S a little pigment, others none. 



In 12 days reaction was acid, all reduced Fehling's 

 solution. 



In 20 days reaction was alkaline, no reduction. 



In 27 days reaction was neutral, strong reduction. 



