[HARRISON & barlow] SLIME-PRODUCING ORGANISM 109 



Character of the Growth. — The liquid became turbid and a ropy 

 white sediment appeared, both the turbidity and the sediment increased. 

 On shaking the culture with a circular motion, the tenacious sediment 

 rose in the liquid and twisted or coiled on itself in a spiral column. It 

 diffused on persistent shaking and settled out on standing. The above 

 description is applicable to all cultures observed in transparent liquid 

 media. 



None of the cultures of this series became so slimy as to draw our 

 in threads. The bouillon was not the most favorable liquid medium 

 for growth. 



Color appeared only in the 1/10'i and the 1^ and only at 18° to 

 22° C. It appeared in 6 days and disappeared in 20 days, passing 

 through the green-blue pigmentation. The bouillon was unfavorable 

 for pigment formation even with the sucrose. 



Sucrose was inverted at all temperatures and in all the concentra- 

 tions, except 1/10^/, the most favorable concentrations were 10^, 1^ 

 and 20^. 



Growth in Lactose Bouillon. — Beef peptone bouillon with lactose 

 0^, 1/10^, 1^, 10^ and 15^. Growth took place at all temperatures 

 between 4° C. and 37° C. Lactose 10;^ and 15^ retarded growth moro 

 or less. In 13 days, there was strong turbidity and much ropy sediment 

 in all tubes of this series. In 35 days the 1/10;?^ and 10<^ were so slimy 

 as to draw out in threads. Other cultures of this series became viscid 

 but not so slimy as these. x\t the higher temperatures there was a ten- 

 dency to form growth on the sides of the tubes. This bouillon was not 

 the most favorable liquid medium for growth or pigmentation. A pale 

 green-blue pigment developed at 18 to 22° C. in the 1/10;^ in 8 days and 

 in the 1^ in 25 days. 



Dunham's Solution. — In 2-1 hours at 18° to 22° C. or at 25 — 

 27° C. there was a little turbidity, increasing, with a ropy sediment, in 

 2 days. A faint gTcen-blue tint appeared in 5 to 9 days; it increased a 

 little and in 22 days faded out, leaving the medium amber-colored. The 

 cultures became viscid and at length so slimy as to draw out in threads. 

 This took place in 6 days at 25° to 27° C. but required more time at 

 18° to 22° C. 



Dunlianis Solution with Sucrose 5^. — A tube was inoculated and 

 kept at room temperature and first observed after 4 days. It was then 

 turbid and so slimy as to draw out in threads. It had developed a green- 

 blue color. There was inversion of the sucrose. In 6 hours after the 

 first observation the culture was visibly darker and there was a percep- 

 tible daily increase in color for 3 more days. The culture remained dark 

 green-blue for some days but after 25 days the color began to fade io 



