[HARRISON & barlow] SLIME-PRODUCING ORGANISM 111 



odor and on testing the hot milk after boiling the acidity was found to 

 be -f 35°. 



Milh Tubes at 18° to 23° C. — Growth in test tubes was much the 

 same as in flasks. The maximum pigmentation varied in different tubes 

 from light blue to bright blue and even to dark blue, the more intense 

 the color the more narrow the colored layer. In 6 days the peptonized 

 layer was 2 m.m. deep and deeper in 11 days. In 11 days some tubes 

 showed coagulation on boiling. 



Milk Flasks at 25° to 27° C. — The changes were more rapid than 

 at lower temperatures. Inoculated with one loopful the milk became 

 slimy throughout, or at least at the bottom, in 24 hours, and very slimy 

 in two days. In 1 or 2 days a diffuse green-blue color appeared in the 

 upper half of the milk, changed to light bine and began to fade in 5 to 

 6 days. In 8 days nearly all the color was gone. The peptonized layer 

 was 5 m.m. deep in 5 days and 10 m.m. in 8 days. In 9 days there 

 was a soft curd, partially separated, and the acidity was -)- ^2°. In 16 

 days the acidit}^ was -|- 28°, the curd had settled and the amber colored 

 whey was thick and slimy. In 26 days the curd was not wholly digested, 

 the acidity was -(-52° in one flask and -[-90° in a second flask. Stains 

 were made and the cultures were apparently pure. Complete digestion 

 of the curd and loss of sliminess have been observed in old milk cultures. 



Milk Tubes at 25° to 27° C. — Growth in tubes was slower than 

 in flasks. In 9 days there was peptonization but no coagulation; m 

 10 days the curd separated in fine flakes on boiling; in 16 days there 

 was complete coagulation, the curd was amber to ochre-yellow and the 

 cultures acid -|- 28°. Litmus was more rapidly reduced than at 18° 

 to 22° C. 



Milk at 37° C. — With ordinary inoculation it was difiicult to obtain 

 growth in milk at 37° C, but growth followed liberal inoculation. The 

 cultures became slimy and the curd separated in 2 days; there was no 

 pigment. 



A flask was incubated at 25 to 27° C. until a sky blue laj'-er 

 appeared. It was then put at 37° C. and in 8 hours most of the color 

 was gone. All color disappeared in 24 hours but on incubation at the 

 lower temperature the blue color returned. This was repeated, using 

 the same culture and with the same results. 



There was no growth in milk at 38.2° C. 



Growth in Whey and Peptone Whey. — Whey acid +18° was 

 partly clarified by steaming and straining. It was then heated an hour 

 with CaCo., in excess of the acidity, i. e., 2 grams per litre. It clarified 

 and the filtrate was greenish yellow, bright and clear and acid -|- 11° 

 Peptone 1^ was added to a part which was then heated and filtered. 



