[HARRISON & BARLO-w] SLIME-PRODUCING ORGANISM 113 



At 18° to 22° C. — There was growth in all, most in Uschinsky''? 

 medium. In 2 days all were turbid with ropy white sediment, but there 

 was more growth in Uschinsky^s medium and Cohn's than in Fermi's 

 medium. Growth increased in this order for 10 days and Usehinsky's 

 and Cohn's media became viscid but did not draw out in threads. 



At 25° to 27° C. — In 24 hours there was good growth in Cohn's 

 and Usehinsky's, none in Fermi's medium. In 2 days all were turbid 

 with an abundant ropy white sediment in Cohn's and but little in 

 Fermi's medium. At length, however, there was more growth in Fermi's 

 medium so that it alone could be drawn out in slimy threads. 



At 37° C. — There was growth in 24 hours in Usehinsky's medium, 

 which increased for two days, no growth in the others. In 8 days the 

 growth had increased in Usehinsky's medium and there was a slight 

 growth in the others. The growth in Fermi's medium was in the form 

 of little white dots or specks in the liquid. At 38° C. there was a slight 

 growth in Usehinsky's medium only. 



Oxygen Requirements. — Two sets of media were inoculated. The 

 tubes of one set were put aside at room temperature and the others were 

 put in a Novy jar and a stream of purified hydrogen was passed through 

 the jar for 2 hours, after which the jar was sealed and set aside a"^ 

 room temperature. 



The tubes in the air of the room developed their characteristic 

 slimy and pigmented growths. Thus, the potato became bright blue in 

 24 hours, the culture in 1^ peptone and 5^ sucrose in tap water slowly 

 became green-blue and so slimy as to draw out in long threads, and the 

 culture on plain agar was slimy, without pigment formation. 



After 3 days the Novy jar was opened. There was growth in the 

 control tubes inoculated with colon bacillus, but there was no gro\vth 

 in the tubes inoculated with the blue slimy organism. The organism 

 was not dead, however, for on removal to the air, the potato tube 

 developed growth and a bright blue pigment in 24 hours, and the cultures 

 on agar alkaline — 5° sucrose and in Dunham's solution and Dunham's 

 solution -f- sucrose 5^ developed their characteristic slimy growth and 

 pigment. 



Conclusion. — The organism does not grow in an atmosphere of 

 pure hydrogen. * 



Growth on agars in test tubes closed with sealing-wax. — The 

 medium was beef extract peptone agar modified with lactose 1/10^^, 

 1^, 5^, 10^, 20^, and 25^. The tubes were of about 20 c. c. capacity 

 and contained either 5 c. c. or 10 c. c. of the slanted agar. All were 



Sec. IV., 1905. 8 



