[HARRISON & barlow] SLIME-PRODUCING ORGANISM 



115 



In the lactose bouillon there was more growth than in the others 

 but no gas formed even in 35 days. The growth was confined to the arm 

 and to the connecting tube and was sharply separated from the open 

 clear liqud in the closed arm. The growth soon became so slimy as 

 to draw out in long threads and then thickened to a mucus-like mass 

 Color appeared in 5 or 6 days and the liquid in the open arm passed 

 through the deep blue pigmentation. In 30 days the blue was replaced 

 by ochre-yellow except at the surface and in 35 days it was wholly 

 destroyed and changed to a rust-color which diffused upward into the 

 clear liquid. 



Beside these cultures in fermentation tubes cultures were made 

 in liquid media and stab cultures in gelatines and agars with various 

 carbohydrates as described elsewhere, but gas formation was never 

 observed in cultures of this organism. 



Effect of Carholiydrates of different concentrations on growth and 

 pigmentation. — Beef peptone agar, 3° acid to phenolphthalein, was 

 modified by adding various amounts of carbohydraies as given in the 

 table below. All the media was sterilized by the discontinuous method 

 at 100° C. The alcohol was sterilized in a pipette and added to the 

 melted and sterilized agar. 



Carbohydrate. 



Alcohol ; 



Glycerin 



Mannite 



Maltose 



Galactose 



Dextrose 



Lactose 



Sucrose 



Dextrin 



Starch 



Lévulose 



Gum Tragacanth 



Percentage of Carbohydrate Added. 



The tubes were inclined and were inoculated by drawing a needle 

 dipped in a bouillon culture over the inclined surface of the agar. 

 The cultures were incultated at 19 — 22° C, the temperature being 

 20 °C. most of the time. All the cultures were under observation for 

 22 da3's and some of the:n for 50 days or longer. 



