124 ROYAL SOCIETY OF CANADA 



Viability in Various Media at Different Temperatures. 



Effect of Decinormal Sodium Hydrate on Growth and Pigment- 

 ation. — Decinormal sodium hydrate w^as added to tubes of melted sucrose 

 agar after sterilization in quantities indicated by alkalinities, — 10°, 

 — 20°, — 40°. The tubes were inclined, inoculated and incubated at 

 18° to 22° C. 



There was good growth in all the tubes, though — 30° was at first 

 a little retarded. All became richly pigmented. The maximum of 

 pigmentation was delayed in — 20°, but the period of rich pigmentation 

 was lengthened and the amount of pigment was increased in proportion 

 as the alkalinity increased; thus, — ±° sucrose agar was riclily pigmented 

 for 9 days,— 10° for 13 days and— 20° for 35 days. 



Effect of Sodium Chloride. — To 1^ of peptone in tap water was 

 added NaCl 0^, 1^, 2^, 3^, 4^, 6^, and the tubes were sterilized m 

 steam on three successive days, inoculated and incubated at 25° to 27° C. 



In 4 days there was growth and all were nearly equally turbid with 

 a ropy sediment. In 9 days and in 15 days the tubes were shaken until 

 the slimy sediment diffused and the culture with no NaCl was so slimy 

 as to draw out in threads. None of the others were so slimy. 



Some green-blue pigment appeared in the cultures 0^, 1^, 2^ and 

 3^ in 9 days and in 4^ in 15 days, none in the 5^ in 20 days. 



The series was continued in the same manner with NaCl 6^, 7j^, 

 8^, 9^ and 10^. In 3 days there was growth in 6^ and 7^, in 9 days 

 there was growth in 8^ but after 14 days there was no growth in 9^ nor 

 in 10^. There was no pigmentaton in these cultures. The turbidity 



