42 U. S. BUREAU OF FISHEEIES. 



line. No sand being available for the purpose, cinders were sub- 

 stituted, and to the presence of this material is attributed the loss of 

 approximately 46,000,000 of the first eggs received. The removal of 

 the cinders and the refilling of the filter with sand and gravel cor- 

 rected the situation, though the output of the substation was consid- 

 erably reduced. 



The first eggs were taken on February 27, and during the spawn- 

 ing season, which ended on March 25, a total of 96,440,000 eggs were 

 taken. Of these, 1,440,000 were planted on the spawning grounds 

 after being fertilized and 38,600,000 fry were developed at the 

 hatchery. The conditions were unusual, inasmuch as the Atacha- 

 falaya River was at a low stage until late in January, whereas a rise 

 occurs in the late fall in more normal years, its effects continuing 

 into the spring months. To this condition may be attributed the 

 short season and the small numbers of fish on the customary spawn- 

 ing grounds. The water temperatures during the egg-collecting 

 period ranged from 60 to 76° F. The incubation period was 10 

 days in a mean water temperature of 60°. The eye spots in the egg 

 are visible to the unaided eye in from four to five days, and the fry 

 have absorbed the umbilical sac in from four to five days after 

 hatching. 



Further counts and measurements to ascertain the correct number 

 of eggs to a liquid quart were made and a standard of 120,000 was 

 established as a result. Observations were made at several points 

 to determine the percentage of fertilization that is attained in eggs 

 deposited under natural conditions. The results varied from zero 

 in certain places to as high as 40 per cent at others, with an average 

 of 10 per cent at all points considered. Efforts were also made to 

 secure information relative to the spawning of the paddlefish, but 

 nothing of a reliable nature was gained. 



Buff'alofish jjropagation conducted on an experimental scale at 

 Clarendon, Ark., has been permanently discontinued, it having been 

 found that very few of the local fishermen are inclined to lend their 

 cooperation. 



PROPAGATION OF ANADROMOUS FISHES OF ATLANTIC COASTAL 



STREAMS. 



Included under this head are the shad, glut herring, alewife, 

 striped bass or rockfish, and the Atlantic salmon. 



PROPAGATION OF SHAD, BRYANS POIXT (m1).) AND EDENTON (n. C.) 



STATIONS. 



The work addressed to the shad is conducted at only two hatch- 

 eries, Bryans l*oint, on the Potomac River in Maryland, and Eden- 

 ton, on All)emarle Sound in North Carolina. It is interesting to 

 note that the run of shad is being maintained in these regions, not- 

 withstanding the failure to put into effect the greatly needed regula- 

 tory measures for which the bureau has worked. During the 12- 

 year period from 1910 to 1921, both fiscal years inclusive, the Mary- 

 land hatchery distributed a total of 448,799,000 shad fry, an average 

 annual output of 37.399,000. The total output of shad from the 

 North Carolina hatchery during the same period amounted to 



