COMPOSITION OF MICROBIAL AMINO ACID POOLS SI 
TABLE V 
AMINO ACID POOLS IN CORYNEBACTERIUM, MYCOBACTERIUM AND RELATED ORGANISMS* 
Mycobacterium Mycobacterium = tbsp 
: tuberculosis tuberculosis orynebacterium Actinomyces Nocardi 
Organism : . eee diphtheriae el y é pane a 
var. hominis var. hominis PW-8 phaeochromogens rugosa 
H37k\ H 37K) 
Ref. numbers 141 51 188 14 10 
Growth Medium** (CS) eS SO és CO 
Age (days) 21 20 Gi 3 
Glutamic acid e@ ® e@ C°) e@ 
Aspartic acid ®@ @ S e@ © 
Glutamine @ 
Asparagine 
Alanine ® @ @ @ ® 
Glycine ® e@ @ ® e@ 
Threonine e@ C ) ® 
Serine e@ ® 
Lysine ia) ie) @ e@ 
Hydroxylysine @ 
Arginine ® @ fo) 
Histidine ® 
Leucines Q e@ @ @ e@ 
Valine @ @ ie) @ @ 
Methionine e@ ® 
Proline Q C3) 
Tyrosine Qa @ (a) 
Phenylalanine @ é 
Tryptophane 
y-Aminobutyric acid @ eo) 
p-Alanine @ 
a-Aminobutyric ® @ 
Cyst(e)ine e@ ® 
Diaminopimelic acid © 
Unknowns @ @ 

* See footnote * Table I. 
** See footnote ** Table I. 
The bacterial spore with its unique physiological properties has been examined by 
a number of investigators. The study of PFENNIG on B. subtilis spores already has 
been cited (Table I, ref. 142) to show that the pool is less varied and about one fifth 
the size of the vegetative cell pool. Dipicolinic acid (pyridine-2,6-dicarboxylic acid) 
has a unique occurrence in bacterial spores (cf. ref. 145) and considerable interest in 
its relation to the germination process and heat resistance of the spore®* has developed. 
Dipicolinic acid appears on the basis of recent studies to be in the free state within the 
mature spore. A number of studies have shown, for example, that mechanical dis- 
ruption as well as other procedures such as electrodialysis and treatment with deter- 
gents all release dipicolinic acid in a soluble, apparently uncombined form! 19°, 19, 
YounG™ also observed release by mechanical disruption and hot water extraction, 
but her chromatographic findings suggest that this substance may occur in a combined 
form. 
References p. 105/108 
