84 J. T. HOLDEN 
TABLE VII 
AMINO ACID POOLS IN MOLDS* 




— S = 
ses S S38 =§ <i g 
$28 QS Ses S 5 = = 
Organism g — $ R = ges & = 2 So 5 
Soe S SoS aS 5 a 
q r= Zz qs Se x 
Ref. numbers 55 136 138 93 147 162 143 
Growth Medium** CS DO GS — CO DO S 
Age (days) 5 2 3 — 2 3 5 
Glutamic acid 0.70 4.5 0.50 5.8 AA @ Be7 
Aspartic acid 0.09 1.9 0.05 1.9 AA @ 3.0 
Glutamine ’ 0.50 22 A @ 
Asparagine 0.67 
Alanine 1.85 6.2 3.40 8.4 AAA ® 9.9 
Glycine 0.12 2.6 0.36 5.1 AAA *** @ 1.9 
Threonine 0.29 2.6 0.38 282 1.5 
Serine 1.25 2.6 0.37 14.3 8 1.4 
Lysine 0.32 2.4 0.19 2.6 (=) 251. 
Ornithine AS e 
Arginine O.11 2.9 A 2.0 
Histidine 0.10 ely A r 
Leucine/ Isoleucine 0.688§ 3.7 0.68 6.5/9.5 AA @ 1.8/1.3 
Valine 0.48 32 0.45 12.8 A @Sss 2.9 
Methionine 0.47 2.6 @ 
Proline 0.04 7-4 A 2 19/ 
Tyrosine 0.09 1.5 0.21 0.4 1.3 
Phenylalanine 0.20 1.5 @ 0.7 
Tryptophane 7.4 0.5 
y-Aminobutyric acid 0.05 1.30 ®@ 22 
p-Alanine 0.18 
a-Aminobutyric acid 0.17 
Cyst(e)ine @ 2.9 
Cysteic acid 0.17 ® 
Glutathione 0.31 
Unknown 2 TEE 

See rtootmote abled: 
** See footnote ** Table I. 
*** Reported as glycine and/or serine. 
§ Reported as ornithine and/or lysine. 
§§ Reported as leucine—phenylalanine. 
§§§ Reported as valine—methionine. 
were encountered in all the strains studied including taurine which is rarely found in 
microorganisms. As can be seen in Table VII, individual strains did not contain all 
these substances. In the other report on Neurospora shown in Table VII, the mold 
was grown in media containing one of several amino acids. Although fewer amino 
acids were reported (one-dimensional chromatography) all of them were present at 
levels at least twice as high as those reported by FUERST AND WAGNER. The latter 
authors did investigate the effect on the wild type pattern of growing the mold with 
single amino acids, purines and vitamins, and observed both increases and decreases 
in levels of individual amino acids. Conidia of Neurospora sitophila have been examined 
References p. 105/108 
