FREE AMINO ACIDS IN PROTOZOA Telok 

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BUTANOL-~ ACETIC ACID-WATER (4:1: 1) 
Fig. 1. Diagram showing Rp values of amino acids and related substances in phenol-water and 
butanol-acetic acid—water systems. This is the standard for identification of spots in Figs. 2-6. 
Abbreviations refer to amino acids and related compounds, including unknowns referred to in 
Table I. 
on chromatograms of the FAAs of strain GL (SCHERBAUM e¢ al.?). Correlations between 
spot size and actual amounts of eluted materials enabled us to devise a four-category 
semi-quantitative scheme for indicating relative amounts of FAAs observed on the 
original chromatograms. This scheme is utilized in the comparisons of FAAs shown 
in Tables I and II. 
Except for the sulfur-containing amino acids cystine, cysteine and cysteic acid, 
taurine and the unknowns, there is little variation in the size and color intensity of 
spots between material obtained from the several strains of Tetrahymena tested. 
Neither are there striking differences in spot size evident between the several cultures 
incubated at 10° and 35°. In all cases 14 ninhydrin spots, probably representing 18 
FAAs were observed, with additional S-containing amino acids and taurine in cases 
as noted below. 
The FAA variations observed on chromatograms among the eight strains of Tetra- 
hymena studied with respect to the S-containing amino acids, cystine, cysteine, 
cysteic acid, taurine and several unknowns are compared in Table II. When these 
three sets of spots for each strain were compared, no two were quite alike, except 
References p. 114 
