160 J. B. LOEFER AND O. H. SCHERBAUM 
TABLE: 
AMINO ACIDS AND RELATED COMPOUNDS IN TETRAHYMENA 

Modified from LOEFER AND SCHERBAUM}. 
Key to relative color intensity of spots: 
+-+, very strong; 
+, strong; 
+, low concentration; 
—, very weak or absent; 
F these sulfur-containing substances and ‘“‘unknowns”’ are the variables among the strains 
) 











shown. 
Regular patterns* These strains had additional ninhydrin-positive spots as shown below 
(These 14 (These rg _ a _ 
, Vi Lay ey WH52 AS 
Substance ( o all es all Eis ie ae 2 = 
strains) strains) : TOD | 25r Neo 5 aeenOe AG Sie 
PAA FAA 
to Misc. AAs*¥* + + ++ 
4 Misc. AAs*** +--+ ar 
Asparagine — = 
Cysteine/cystine + () — =e ar te + 3 = ar es ae 
Cysteic acid aE () Se a2) Se 
Glutamine — + 
Proline ae == 
Taurine == () ++ 4 Pe 
Tyrosine = aa 
Unknowns: X, a () == sPor SFr GF SS SSP ae ae 
Xs = () inate = —i == 
Xs — () + 

* All data for 25° cultures, except where indicated for WH52 and HS. 
** This group of ten included: alanine, glutamic acid, glycine, leucine/isoleucine/phenylalanine, 
serine, threonine, valine/methionine. 
*** This group of four included: arginine, aspartic acid, lysine/histidine. 
those for T. limacis and strains Gf-J and Li of 7. pyriformis, in which strains all of 
these spots were very weak or absent. In all cases cystine, cysteine and cysteic acid 
occurred in relatively low concentrations, as compared with most of the other sub- 
stances. The failure to detect cystine in any of its forms in several strains may be an 
indication, not of its absence, but of the marginal quantity present in these strains. 
In three strains at 35° (F, L3 and PR) taurine was quite abundant, the spot intensity 
was strong in strain HS at 10°, but very weak or absent in the others. 
One unknown, Xz, was observed only in WH52-10° culture chromatograms (Fig. 5) ; 
another, X,, in strains L3, PR, F and HS at 25° (Figs. 2 and 3) and in HS-ro° and 
WH52-35° material (Table II). 
The unknown X,, was detected in 10° cultures of HS and WH52 (Fig. 5). 
Fig. 6 is a photograph of a chromatogram of GL-29° 2-day stationary phase material. 
Inspection reveals the unknown X,, seen in Fig. 5, as well as another, which has been 
designated X,. SCHERBAUM ef al.®? observed an unknown corresponding to the latter 
in culture samples collected just prior to the beginning of synchronous division. 
When aliquots of the material used for spotting the chromatogram shown in Fig. 6 
References p. 114 
