Mad 4 
_T. PYRIFORMIS HS- 25°C. 
i) 5 i pul 

T. PYRIFORMIS WH52-25°C.#@ T. PYRIFORMIS WH52-10°C. 
4 | ' 
Fig. 4 Fig. 5 
were hydrolyzed prior to spotting, unknowns X, and X, were no longer detectable. 
It would appear, therefore, that unknowns X, and X, represent peptides. The precise 
nature of these four unknown substances requires further investigation. 
The semi-quantitative scheme used above was extended to include comparisons 
with the findings of Wu AnD Hoacc?. The latter employed both chromatography and 
microbiological methods. Their method revealed the presence of tryptophane, not 
shown by others. Their method also throws light on the relative amounts of amino 
Reference sp. I14 
