It4 J. B. LOEFER AND O. H. SCHERBAUM 
cy. a 
6. T. PYRIFORMIS GL- 29°C: 
Fig. 6 

acids that may be present in some of our combined determinations. For instance, 
valine is probably predominantly responsible for the valine—methionine spot; and 
lysine for the lysine—histidine spot. Except in Figs. 2 and 6 (strains F and GL), histidine 
appears to account for a relatively small portion of the lysine-histidine spot. 
CHRISTENSSON®, using synchronized cells and one-dimensional chromatography, 
obtained quantitative results of the same comparative order as ours. He did not 
report the occurrence of taurine, asparagine or glutamine, which were found in strain 
GL by SCHERBAUM et al.?. 
Other strains of Tetrahymena (Table I) analyzed by SCHLEICHER® and WELLS’, al- 
though not comparable in our semi-quantitative scheme, nevertheless reveal a quali- 
tative array of FAAs basically in agreement with those reported above. 
Although factual differences noted in eight reports for protozoa on the occurrence 
of FAAs and related compounds appear to be evident from Tables I and II, particularly 
among strains of Tetvahymena, any conclusion as to the significance of these differences 
would at the present time appear to be premature. 
REFERENCES 
1 J. B. LoEFER anp O. H. ScHERBAUM, J. Protozool., 8 (1961) 184. 
2 J. B. LOEFER anv O. H. SCHERBAUM, Anat. Record, 131 (1958) 571. 
3 O. H. SCHERBAUM, T. W. JAMES AND T. L. JAHN, J. Cellular Comp. Physiol., 53 (1959) 119. 
*C. Wu anv J. F. Hoce, Arch. Biochem. Biophys., 62 (1956) 70. 
5 E. G. CHRISTENSSON, Acta Physiol. Scand., 45 (1959) 339. 
6 J. D’A. SCHLEICHER, Paperchromatography Analyses of Amino Acids in Protozoa: Some As- 
pects of the Metabolism of Aspartic Acid (Cath. Univ. Amer. Biol. Studies 53), Catholic Uni- 
versity of America Press, Washington, 1959, 74 pp- 
UnGe WELLS, Ife Pyvotozool., 7/ (1960) 7: 
8H. E. FINLEY AND H. B. Wiixiams, J. Protozool., 1 (19: 
9 J. W. LEE AND A. RENE, Proc. La. Acad. Sct., 18 (1955 
