I20 Pp. S. CHEN 
nauticus, CLARK AND BALL*® also found these amino acids, except proline, /-alanine 
and asparagine. They included, however, arginine, cysteic acid, histidine, hydroxy- 
proline and phenylalanine. More recently, Po-CHEDLEyY!” reported in the blood of the 
oriental beetle Anomala orientalis the presence of a-amino-n-butyric acid, arginine, 
cystine, histidine, ornithine, phenylalanine and taurine in addition to those found 
for E. varivestis. 
Orthoptera 
CLARK AND BALL* recorded in the adult blood of the oriental cockroach Blatta orien- 
talis the occurrence of Ig amino acids including cysteic acid (Table 1). AUCLAIR AND 
DUBREUIL? in addition found lysine, which occurs however at a low level. 
Adults of the American roach Periplaneta americana contain the following free 
amino acids: alanine, cystine, glutamic acid, glycine, isoleucine/leucine, methionine, 
proline, serine, tyrosine, valine and glutamine (PRraATT!*?). This insect apparently 
has fewer amino acids than the German cockroach Blatella germanica (see Table 
I). 
In adult females of the stick insect Dixippus morosus, DRILHON® estimated the 
relative proportion of amino acid concentration as follows: valine, glycine, serine, 
tyrosine, leucine. DUCHATEAU, SARLET AND FLORKIN®! detected in addition, alanine, 
lysine, methionine, phenylalanine, proline and threonine. Among these, histidine 
occurs at the highest level. 
According to the recent work of TREHERNE!” the concentration of glycine and 
serine in the hemolymph of adult females of the locust Schistocerca gregaria 1s particu- 
larly high. Certain amino acids such as leucine, alanine and valine show an especially 
high degree of variation. 
Odonata 
In the hemolymph of the dragon-fly nymph Aeschna cyanea, RAPER AND SHAW? 
estimated that glycine, alanine, valine and leucine have the greatest concentration, 
whereas serine and lysine are present in small quantities (Table 1). DuCHATEAU AND 
FLorkin®® found, in addition, aspartic acid, glutamic acid, histidine, methionine, 
phenylalanine and threonine. The latter authors did not identify the species in- 
vestigated. 
Hymenoptera 
Patterns of amino acids in the blood of worker, drone and queen larvae of the honey 
bee Apis mellifera have been analyzed by PRratt!2. Histidine and a-amino-m-butyric 
acid were found only in the queen larvae. DUCHATEAU, SARLET AND FLORKIN® deter- 
mined in the bee larvae the concentration of 15 amino acids among which glutamic 
acid, lysine and proline occur at a markedly high level. It is known that the worker and 
drone larvae are fed on pollen, whereas the queen larvae on royal jelly. AUCLAIR AND 
JAMIESON" reported 21 different free amino acids including histidine and a-amino-n- 
butyric acid in several pollen species. But PRATT AND House? included no cystine, 
histidine, hydroxyproline, phenylalanine, threonine and tryptophane in the list of 
free amino acids found by them in the royal jelly. Further studies are needed to clarify 
such discrepancies. KALLHAMMER!® found in the hydrolyzates of young bees at 
hatching 15 amino acids including phenylalanine. Tyrosine showed a large variation. 
References p. 13 |135 
