FREE AMINO ACIDS OF MARINE INVERTEBRATES 183 
Chromatograms of extracts from various tissues of the green abalone, Haliotis 
fulgens, illustrate the differences between the various tissues in this species. The 
white muscle (Fig. 29) had large amounts of taurine and arginine. The male gonadal 
tissues (Fig. 30) were extremely rich in taurine and glycine, while glutamine was not 
evident at all on the chromatograms. Taurine was the major ninhydrin-reactive 
constituent detected in the digestive diverticulum (Fig. 31). 
Amino acid patterns of various tissues of the octopus, Octopus bimaculatus, in this 
case illustrating considerable constancy from one tissue to another (Figs. 32-34), 
show a high level of taurine and the presence of an unidentified compound near glutamic 
acid which gives a yellow color with ninhydrin. Other tissues showed different patterns: 
the brain and eyes had high levels of aspartic acid (which also was high in the lobster 
nerve); the gonads of glutamine; the ink glands of valine, the leucines and tyrosine ; 
the digestive glands of arginine. 
29° ane 
+ 

Figs. 29-34. Green abalone, Haliotis fulgens. Fig. 29, muscle; Fig. 30, male gonadal tissues; Fig. 31, 
digestive diverticulum. Octopus, Octopus bimaculutus. Fig. 32, leg muscle; Fig. 33, gill; Fig. 34, 
excretory tissue. Taurine, 5; glycine, 14; arginine, 15; unknown amino acid, Z. 
References p. 186 
