186 J. S. KITTREDGE ef al. 
acid distribution in marine invertebrates, illustrates the necessity of considering several 
criteria in any approach to comparative biochemistry. (1) Adequate taxonomy. 
(2) Phylogenetic relations of specimens. (3) Ecological influences. (4) Developmental 
state. (5) Comparative morphology. -(6) Sampling of individual tissues. (7) Bio- 
chemical techniques that will permit the recognition of new factors. 
ACKNOWLEDGEMENT 
This work was supported in part by grant No. 3001 (00) from the Office of Naval 
Research. 
ADDENDUM 
The new amino acid occurring in the Coelenterata (“X”, Fig. 7, 8, g and 10) has 
now been identified as 2-aminoethyl phosphonic acid and “Y” has tentatively been 
identified as its glycerol ester. This phosphonic acid, previously isolated from the 
hydrolyzed “proteolipid-like” material extracted from sheep rumen ciliates*4, is the 
first compound containing a phosphorus to carbon bond reported from biological 
material. 
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