BODY FLUIDS AND EFFECT OF HEREDITARY DISORDERS 197 
PR 
7 
PHE YA 
Leu 
+ 
WEU 
Thu 

PRO 

guy 
ARG 

4 
Fig. 1. A paper chromatogram of normal human blood plasma (625 jl).* 
there are a number of others, usually found in the plasma in lower concentration, 
which are normally present. The most conspicuous of these are taurine, ornithine, 
a-aminobutyric acid and citrulline. There are others also which are found abundantly 
in urine but which have plasma clearances which are high, or are suspected to be high, 
and their concentration in the plasma is extremely low. The 1- and 3-methylhistidines, 
f-aminoisobutyric acid as well as the dipeptides carnosine and anserine are in this 
category. 
With regard to the blood plasma amino acids of other vertebrates very little is 
of the sample. Ist solvent (from right to left) water-saturated phenol used in an ammoniacal 
atmosphere (several drops of concentrated ammonia solution put in the tank before sealing). 
2nd solvent (from bottom to top) lutidine (commercial grade, a mixture of the 2,4- and 2,5-dimethyl- 
pyridines). The solvent is made up by mixing 2.2 parts of lutidine with 1 part of water (v/v). 
Kev to the abbreviations on the chromatograms: ABA, a-aminobutyric acid; ALA, alanine; ARG, 
arginine; ASA, argininosuccinic acid; ASA-B, anhydride form of ASA; ASP, aspartic acid; BAIB, 
p-aminoisobutyric acid; CIT, citrulline; CYS-A, cysteic acid; CYSTA, cystathionine; ETH-NH,g, 
ethanolamine; ETH-PO,, ethanolamine phosphate; GLU, glutamic acid; GLU-NH,, glutamine; 
GLY, glycine; HIS, histidine; ILEU, isoleucine; LEU, leucine; LYS, lysine; METH, methionine; 
ORN, ornithine; PHE, phenylalanine; PRO, proline; SER, serine; TAU, taurine; THR, threonine ; 
TYR, tyrosine; VAL, valine. 
* Illustrations of paper chromatograms: general remarks. +, marks the point of application 
References p. 217/219 
