BODY FLUIDS AND EFFECT OF HEREDITARY DISORDERS 203 
comprehensive quantitative estimations of the concentration of the various amino 
acids present in normal human C.S.F. have not been reported but WALKER, TELLES AND 
PasTorE”® quote the following mean figures from a series of 26 normal C.S.F. samples: 
aspartic acid, 0.4; glutamic acid, 0.6; serine, 1.6; glycine, 1.0; threonine, 1.3; glutamine, 
7.1; alanine, 1.1; tyrosine, 0.8; valine, 0.7; phenylalanine, 2.1; and leucine, 1.4 wg/ml. 
Peet ~ 
=: Ais. E° Sen 
Be eee 
az my LY 
AB 
ci 
ci. 
Fig. 4. A paper chromatogram of normal human saliva (2.0 ml). 
Saliva. The amino acids of whole saliva have been investigated by many workers (see 
bibliography by RosE AND KERR*). A paper chromatogram obtained from running 
a 2-ml sample of whole saliva, after ultrafiltration and electrolytic desalting, is shown 
in Fig. 4. Once again, it can be seen that the pattern given by the amino acid spots 
is quite different from that given by other body secretions. In particular, GABA seems 
to be present although BERRY AND CaIn* decided that the spot that they found in 
a similar position to that taken up by GABA was, in fact, something else. There is 
also a well-defined spot due to ethanolamine phosphate. Some further interesting 
patterns were obtained by RosE AND KERR*® who ran paper chromatograms on 
secretions, obtained by cannulation, from the parotid and submaxillary glands. They 
found that the concentration of ethanolamine phosphate in these secretions was some 
References p. 217/219 
