204 R. G. WESTALL 
four to five times higher than in whole saliva. This ts curious since it is estimated that the 
secretions from these two glands makes up approx. 75°% of the volume of the whole 
saliva. ROSE AND KERR*® concluded that there must be an enzyme present in whole 
saliva capable of hydrolysing ethanolamine phosphate and which must be absent 
in the parotid and submaxillary gland secretions. This explanation is supported by 
an observation by CHAUNCEY, LIONETTI, WINER AND LISANTI*®, who reported that 
TVK 
oat 
“se LEU 
VAL 
ALA SER 
HIS Wg, ' 
et ASE 
ARG 
LYS 
! 
Fig. 5. A paper chromatogram of normal human sweat (125 wl). 
alkaline phosphatase activity was present in whole saliva. The GABA which is present 
in whole saliva seems to come from some source other than from the parotid or sub- 
maxillary glands*. 
Sweat. The presence of amino acids in sweat has been known for over 50 years when 
EMBDEN AND TACHAU*? managed to isolate serine from human sweat. Fig. 5 shows 
a paper chromatogram of 125 ul of human sweat and it can be noted that serine is 
probably the most abundant amino acid. A similar paper chromatogram to that 
illustrated was published by RoTHMAN AND SULLIVAN’ who obtained their samples 
by wiping down their subjects with water-soaked cotton 24h after bathing. It is 
References p. 217/219 
