BODY FLUIDS AND EFFECT OF HEREDITARY DISORDERS 205 
difficult, from their results, to estimate the amounts of the amino acids which occur 
in the perspiration. However, if droplets of sweat are collected during exercise then 
the amino acid concentration in the sample is found to be about five times that of 
blood plasma. The amino acid pattern of sweat differs from that of other body fluids 
in having a high concentration of citrulline, a substance which occurs in other fluids 
“a 

LYS 
Fig. 6. A paper chromatogram of human tears (250 wl). 
but usually only in trace amounts. Aspartic acid is also found in greater concentration 
in sweat than in the other body fluids. 
Tears. As far as Iam aware very little is known about the amino acids in tears. For the 
purpose of this article a paper chromatogram was prepared using 250 ul of ultra- 
filtered and electrolytically desalted lacrimal fluid and this is illustrated in Fig. 6. It 
will be seen that the amino acid pattern is somewhat similar to that of sweat except 
that the citrulline spot is not as prominent. The amino acid concentration is higher 
than in blood plasma but does not quite reach that of sweat. 
References p. 217/219 
