FREE AMINO ACIDS OF BLOOD AND URINE 225 
cribed!°, The extracts of leucocytes and platelets are concentrated to a few ml, then 
quantitatively transferred in a 10-ml volumetric flask, brought to pH 2.0 and made 
up to volume. Five ml samples are used respectively for 150 and 50 cm ion-exchange 
columns analysis. In the case of leucocytes and platelets, samples of a size suitable 
for each of the two steps of the complete chromatographic analysis are equivalent 
extracts of 100 mg of cells. No attempt at subfractionation of the different types 
of leucocytes has been made here. 
Methods for expressing data on free amino acid content or excretion 
This important point has been discussed at length elsewere!. Here data will be ex- 
pressed mainly on a molar basis, which is more suitable for the sake of comparison. 
Urine free amino acids will be referred to as 24-h outputs. 
FREE AMINO ACIDS OF URINE AND BLOOD PLASMA 
Normal aminoaciduria in healthy adults 
Qualitative composition. A typical elution curve obtained by the MooRE AND STEIN 
1954 manual procedure using unhydrolyzed urine from a normal adult female is 
to be found in a review published in 1959 by BiGwoop e¢ al.1. Fig. 1 shows the auto- 
matically recorded elution curve obtained by use, in our department, of the SPACK- 
MAN, MOORE AND STEIN automatic procedure!! and is in complete agreement with 
that published by these authors. Although the amino acid composition illustrated 
by Fig. 1 is that of urine obtained from an untreated leukemic patient, it 1s given 
in this section in order to avoid duplication of figures since it will also be used in an- 
other section of this report; moreover the amino acid pattern was in this case quite 
normal, both qualitatively and quantitatively. The analytical methods used here 
‘a Urea 
=|) Tourtme 7 
Glyciat 
= 63 
a lek ; Asparagine ne 
sie Serine”  Giatamnine 
3 8 
= Threonine 4 
= A 7 o Alanine 
re eee 5 eS Aspartic a Ghtewie 
> : ond ie: acid 
a + = n i ae ~¥ rat 
Neh x Sie Os, > f 
Eifloent —_ ree SS Neer Ce gE a I 6 Se ee 
Liljatn mi . * » ~ a o = = mt ~ Py a - es oo 
<— F : 
te 
os 
oe . 
és Cyttathtonine a 
| Methivnine ; 
a es 7 | Eavleweume 
| Lewerme Tyrasiat p-amisein- 
ar ~ 
: | Phenylalanine : Lyric” acid 
Valiwe Cystine i 13 ee 

Fig. ta. Chronic lymphocytic leukemic patient’s urine. Determination of acidic and neutral 
amino acids on a 150-cm column (automatic procedure). Free amino acid excretion in this case 
is normal, both qualitatively and quantitatively. 
References p. 261/262 
