232 P. SOUPART 
of plasma appears much simpler and the picture is more clear-cut. There are a 
small number of unidentified ninhydrin-reacting substances and only a few re- 
marks are needed. There are two unknown components, constantly present, eluted 
in the range between that of urea and that of methionine sulfoxides. The gluta- 
mine peak is, in fact, a mixture containing asparagine as well, to the extent of 
ea) 

Optical density 

Efluent mi. 



: ee ag 
eeeReE Cees SeUSSUP SS SC Oevesercersterritrriierrrrr i iii rere Terre eee 
ote OI Re ee a aia AE 



Fig. 4a. Amino acid composition of protein-free human blood plasma from a chronic lymphocytic 
leukemic patient. Determination of acidic and neutral amino acids on a 150-cm column. Free 
amino acid plasma levels are normal in this case. 





= ca Sh = - 50° 
Fig. 4b. Amino acid composition of protein-free human blood plasma from a chronic lymphocytic 
patient. Determination of basic amino acids on a 50-cm column. Free amino acids plasma levels 
are normal in this case, 
References p. 261/262 
