FREE AMINO ACIDS IN ANIMAL TISSUE 207 
of the chromatograms in Figs. 47-58 that there is a pattern of autolytic change 
which is characteristic for each tissue. Separate experiments showed that in no case 
were the amino acids liberated in the same relative amounts during autolysis as 

Figs. 47-58. Changes in free amino acids during sterile autolysis of liver, kidney, muscle and 
brain. Aliquots corresponding to 37.5 mg of fresh weight of tissue were employed. For each 
tissue the numbers are in the following order: control, 8 h autolysis, and 24 h autolysis. Figs. 47-49: 
liver, Figs. 50-52: kidney. Figs. 53-55: muscle. Figs. 56-58: brain. Alanine, 14; arginine, 15; 
lysine, 16; ornithine, 23. 
References p. 348/349 
