FREE AMINO ACIDS IN ANIMAL TISSUE 315 
quantity of which also appeared in the ascitic fluid (Fig. 162). The cytoplasmic and 
nuclear damage increased with time (60-min cells, Fig. 163; 300-min cells, Fig. 166), 
no normal mitotic cells being observed at 300 min. Observations with phase micro- 
scopy showed that damage also occurred to the mitochondria after treatment with 
sarkomycin, the fine granules or thread-like shapes in the untreated tumor cells 
changing progressively after treatment into rounded forms. In spite of the extensive 
damage found in the cells the only remarkable changes in the chromatograms of 
the cells (Figs. 164 and 167) and of the fluid (Figs. 165, 168) were increases in the 
glutamine levels. With the exception of the glutamine content there was a truly 
remarkable similarity between the patterns found in the cells and fluid after treat- 
ment and those of the controls. 


8 
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Ms 
had 
159 
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17 
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Se 162 
“13 165 
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pe... 166] 167 
Figs. 157-168. Photomicrographs of smears ( 500) (Figs. 157, 160, 163, 166) and chromatog- 
rams of extracts of 75 ml of cells (Figs. 158, 161, 164, 167) and 150 yi of fluid (Figs. 159, 162, 
165, 168) of the Yoshida ascites tumor in a Wistar rat before and at 30, 60 and 300 min, re- 
spectively, after the intraperitoneal administration of sarkomycin. Glutamine, 13; alanine, 8; 
glutamic acid, 17. 
References p. 348/349 
