FREE AMINO ACIDS IN ANIMAL TISSUE 319 
Changes in amino acids of tumor cells exposed to high extracellular levels 
of glutamine and glutamic and a, y-diaminobutyric acids 
It is of interest to compare the effects on amino acids of ascites tumor cells of high 
extracellular concentrations of a basic, acidic and neutral substance. Because of the 
virtual absence of free glutamine!: *: 8. 3° from tumor cells it was easy to follow the 
changes of this amino acid after administration to animals bearing ascites tumors*?, 
When 50 mg of glutamine was injected intraperitoneally into rats bearing the Yoshida 
sarcoma, a serial study of extracts of cells and fluid (Figs. 189-202) showed there 
to be a rapid uptake of glutamine by the cells. At the 2-min period the fluid (Fig. 192) 
showed a large quantity of free glutamine. The content of glutamine in the cells and 
fluid decreased progressively after the 5-min period (Figs. 193, 194), the levels in 

Figs. 189-194. For legend see p. 320. 
References p. 348/349 
