FREE AMINO ACIDS IN ANIMAL TISSUE 
WwW 
No 
204 eae 
x 

Figs. 203-208. Chromatograms of extracts of cells and fluid at !/, h (Figs. 203, 204, respectively), 
11/,h (Figs. 205, 206, respectively), 21/,h (Figs. 207, 208, respectively) after intraperitoneal 
injection of 50 mg of L-glutamine under same conditions as in Figs. 189-202. Glutamine, 13. 
the fluid decreasing at a more rapid rate than in the cells. Much larger quantities of 
glutamine were found in the cells obtained at 40 min (Fig. 199) and 55 min (Fig. 201) 
after the injection of glutamine than in the fluid. Throughout this experiment the 
size and morphology of the tumor cells was perfectly normal. The results of an ex- 
periment in which complete disappearance of the injected glutamine from both cells 
and fluid had taken place within 11/,h after the administration of glutamine are 
shown in Figs. 203-208. These results proved conclusively that glutamine enters the 
tumor cells rapidly and then disappears. 
An interesting finding in this experiment was that after the injection of glutamine 
there was a general increase in most of the detectable ninhydrin-reactive constituents 
in the ascitic fluid (Figs. 192, 194, 196, 198), notable increases occurring in contents 
of alanine, glycine, glutamic acid, serine, threonine and lysine. The levels of most 
References p. 348/349 
