FREE AMINO ACIDS IN ANIMAL TISSUE 333 
Studies similar to the one above were made in C57 black/dba female mice im- 
planted with 5 x 108 Ehrlich ascites tumor cells. Samples of tumor, liver, muscle 
kidney, and brain were studied from 48h after transplantation until 11 days, the 
average life span of the tumor-bearing animals. The amino acids in tumor cells and 
ascitic fluid were remarkably constant as were the patterns found in the tissues of 
the tumor-bearing mice. The only changes observed were decreases in glutamine 
levels of both muscle and liver at 8 and 11 days after transplantation. Similarly, 
tissues and tumors were studied in Wistar rats (6-7 weeks of age, 135-142 g) 6 days 
after inoculation into the axillary region with 1 ml of 20° suspension of sterile 
Walker tumor. Tissues of littermates were used for control determination. No 
272 

Figs. 271-278. Free animo acids of brain (75 mg) of rats bearing the Murphy lymphosarcoma 
(ascites) at different times after transplantation of the tumor. Fig. 271 was fiom a control animal 
and Figs. 272-278 were from animals at 6 h and 1, 2, 3, 4, 5 and 8 days, respectively, after tians- 
/ 
plantation. y-Aminobutyric acid, 22. 
References p. 348/349 
