FREE AMINO ACIDS IN ANIMAL TISSUE 335 
which appreciable loss in weight occurred. In one study one group of young Sprague- 
Dawley rats (150-160 g) was deprived of food but given water, while another was 
given food ad libitum but no water. Dehydrated rats were sacrificed at 24, 48 and 
68 h. Results are shown for the starved animals only for the 24- and 48-h periods 
because these animals died before 68h. Between the 48- and 68-h period all ex- 
perimental animals had lost up to 30% of their original body weight. The amino 
acid patterns in brain were not altered in any detectable fashion either by dehy- 
dration or starvation (Figs. 279-284). The kidneys of rats showed slight increases 
in contents of glutamic and aspartic acids and glycerylphosphorylethanolamine 
during dehydration and small increases in cystine, aspartic acid and glycerylphos- 
phorylethanolamine and a decrease in glutamine during inanition (Figs. 285-290). 
In the liver (Figs. 291-296) dehydration resulted in increases in taurine and threo- 
nine levels at all times and in the appearance of an unidentified ninhydrin-reactive 
substance above taurine. In the sample obtained after 68 h of dehydration there 



Figs. 279-284. Amino acids of brains (75-mg aliquots) of dehydrated and starved rats. Fig. 279: 
control. Figs. 280, 282, 284: dehydrated for 24, 48 and 68h, respectively. Fig. 281, 283: starved 
for 24 and 48h, respectively. 
References p. 348/349 
