FREE AMINO ACIDS IN ANIMAL TISSUE 339 

305 306 
Figs. 303-306. Chromatograms of extracts of rat heart and muscle (75 mg). Fig. 303: normal 
rat heart. Fig. 304: rat heart after 6 days inanition. Fig. 305: normal rat leg muscle. Fig. 306: 
rat leg muscle after 6 days inanition. Taurine, 5; alanine, 8; serine, 10; glutamine, 13; glycine, 14; 
glutamic acid, 17; aspartic acid, 18. 
A-deficient rats®! was chosen as an example of the effect of a vitamin deficiency on 
these constituents. Quantitative colorimetric determinations of 15 of the constit- 
uents were made by paper-chromatographic procedures (Table III) in extracts of 
TABEE Tht 
AMINO ACID PATTERNS IN THE TISSUES OF VITAMIN A-DEFICIENT 
AND PAIR-FED RATS®! 

Average values for seven groups of rats 


Amino acids Blood (ug/ml) Liver (ug/g) Kidneys (ug/g) 
A* B A B A B 
Aspartic acid 52 51.6 353 355 995 989 
Glutamic acid 29 28.2 353 358 249 240 
Serine 62.3 60.0 504 506 1094 [108 
Glycine 30.4 31.7 498 498 751 749 
Cystine AEG 147-7 195 IgI 186 182 
Lysine 77-4 79-0 574 579 72: 72 
Histidine 52.1 53-1 244 243 507 563 
Arginine 113 TIO.7 440 439 1770 1765 
Alanine 49 50.2 1175 1168 1756 1753 
Methionine ey 18.5 104 Lo6 96 93 
Valine 28.9 28.8 390.4 395 253 250 
Leucines 16.4 16.2 534-0 530 942 939 
Threonine 40.0 64.0 1469 2089 2453 3041 
Tyrosine 104.5 60.8 1032 808 909 699 
Phenylalanine 88.6 49.8 1469 965 1894 1579 
* A, deficient rats; B, pair-fed normals. 
References p. 348/349 
