FREE AMINO ACIDS IN ANIMAL TISSUE 345 
327 328° 7 329 
se» nn 
* a a th, + 
330 331 { 332 
~ ~ ® 
f 
a * “9. a he “gd oo & m, / 
od ¥ o~ a ee 
So w+ ae - 
9 wa Boce 13 pe 
* ee 6 YS _ ee 
. 336 337 338 
Figs. 327-338. Effect of adrenalectomy and hypophysectomy on amino acids of muscle, brain, 
liver and kidney (aliquots corresponding to 75 mg of fresh weight of tissue). The order of the 
numbers is control, adrenalectomized and hypophysectomized. Figs. 327-329: muscle. Figs. 
330-332: brain. Figs. 333-335: liver. Figs. 336-338: kidney. Ethanolamine phosphate, 19. 
It was of interest then to study the effects of early deprivation of thyroid which 
results in the cretinoid rat. One member of each of three pairs of littermate rats of 
the Birmingham strain was thyroidectomized at birth by a single subcutaneous in- 
jection of 100 wC of carrier-free #11 in the form of sodium iodide dissolved in normal 
saline*. A single subcutaneous injection of sodium iodide (!*8I) was given to littermate 
controls’®. All animals were sacrificed at 32 days of age. There were no significant 
changes in the patterns of the livers of the thyroidectomized animals with the ex- 
ception of an elevation of taurine content in each instance studied. In neither muscle 
nor kidney were there any readily detectable differences between the two groups. 
Particular attention was paid to an examination of the extracts of whole cerebral 
cortex and the gray matter of the cerebral cortex because structural and electro- 
encephalographic abnormalities occur in the brains of the thyroidectomized animals 
* Animals were prepared and tissues sent to us by Dr. J. T. Eayrs, Department of Anatomy, 
University of Birmingham, Birmingham (Great Britain). 
References p. 348/349 
