356 G. ROUSER, B. JELINEK, A. J. SAMUELS, K. KINUGASA 
particularly in leukemia, ethanolamine-O-phosphate may be seen. Phenylalanine, 
tyrosine, histidine, arginine, and lysine are not always observed for the reasons de- 
scribed above. Asparagine cannot always be visualized readily as it tends to migrate 
with glycine. The identification of the spots seen on paper chromatograms of plasma 
samples were confirmed by the methods outlined above. Upon hydrolysis with 6 N 
hydrochloric acid for 18 h at 100° in a sealed tube, the disappearance of glutamine and 
asparagine with the appearance of increased amounts of glutamic and aspartic acids 
were observed in all samples. 
The substances identified on paper chromatograms prepared from different types 
of white blood cells and platelets were: leucine plus isoleucine, valine, proline, alanine, 
TABLE I 
ARTIFICIAL PLASMA AMINO ACID MIXTURE 


Amino acid mg/roo ml Amino acid mg/roo ml 
Alanine 3.0 Lysine 2.6 
a-Amino n-butyric acid 0.2 Methionine 0.5 
Arginine 1.8 Ornithine 0.7 
Asparagine 1.0 Phenylalanine Te?) 
Aspartic acid O.1 Proline 1.9 
Cysteic acid 1.5 Serine 1.2 
Glutamic acid 0.8 Taurine 1.2 
Glutamine 10.0 Threonine 1.8 
Glycine 2.0 Tryptophan Tee 
Histidine 1.3 Tyrosine 1.0 
Isoleucine a9 Valine 2.7 
Leucine 2.0 


threonine, glutamine, glycine, serine, glutamic acid, aspartic acid, taurine, gluta- 
thione and ethanolamine-O-phosphate. Cysteic acid and cysteinylglycine were ob- 
served after oxidation. Cysteinylglycine was not seen regularly in platelets. Some 
samples were observed to contain traces of other compounds such as a-amino 7- 
butyric acid. 
The amino acids seen regularly in erythrocyte samples were aspartic and glutamic 
acids, glycine, alanine, serine, threonine, glutamine, leucine plus isoleucine, valine 
and glutathione. Taurine was present in some samples and occasionally traces of 
ethanolamine-O-phosphate were observed. Small amounts of uncharacterized sub- 
stances were frequently observed. 
Chromatograms prepared from urine samples showed glycine, serine, alanine, 
glutamine, histidine, and taurine as readily visualized and identified spots and valine, 
the leucines, phenylalanine, and tyrosine were usually seen despite some distortion 
of this area of the chromatogram due to the presence of ninhydrin negative material. 
The recognition of tyrosine-O-sulfate, 1-methylhistidine, and 3-methylhistidine as 
constituents of all samples was possible with aliquots equivalent to 0.5 ml or more 
of urine. The exact correspondence of migration of added authentic tyrosine-O- 
sulfate (obtained from H. H. TALLAN) with the spot on urine chromatograms giving 
rise to tyrosine and sulfate on hydrolysis is shown in Figs. 11 and 12. 
References p. 447/448 
