374 G. ROUSER et al. 
showed an elevation of all of the amino acids with a particularly large elevation of 
the glutamic acid level. This patient (L. Gre. 2) with polycythemia of several years 
standing had not been treated (other than periodic blood withdrawal) and at the 
time of examination had a white count of 30 000/mm and a thrombocytosis. Almost 
all (95°) of the white cells in the peripheral blood were neutrophilic polymor- 
phonuclear leukocytes. The more extreme cases shown in Figs. 77 and 78 are to be 
compared with the results from other patients (see parts V and VI). A rather marked 
elevation of the plasma glutamic acid level is shown for a patient (H. Gol. 3) with 
granulocytic leukemia in the first part of this series (Figs. 32-34). 
Aspartic acid is not detectible on the chromatograms shown in Figs. 76-78. As- 
partic acid was not observed on a number of chromatograms from normal plasma, 
and was frequently absent from the plasma samples obtained from patients with 
chronic granulocytic leukemia, particularly after drug administration with a reduc- 
tion in the leukocyte count (see part V). Aspartic acid in normal plasma is illustrated 
in part VI (Figs. 268-277, 304-308 and 314-318). 
Platelets and leukocytes 
Fig. 79 shows a normal platelet free amino acid pattern (subject E. Eme.), while 
Figs. 80 and 81 show the platelet free amino acids from the two patients (E. McG. 
and L. Gre.) whose plasmas were presented in Figs. 77 and 78. Fig. 82 shows the 
leukocyte free amino acid pool from the same normal individual (E. Eme.), while 
Fig. 83 (patient E. McG.) and Fig. 84 (patient L. Gre.) are chromatograms from 
leukocytes of the patients whose plasma and platelet free amino acids are illustrated 
(Figs. 77, 78, 80, 81). By comparison of Figs. 79 and 82 (as well as 80 and 83, and 
81 and 84) it is evident that blood platelets and neutrophilic polymorphonuclear 
leukocytes from the same blood samples have similar free amino acid patterns. The 
platelets and leukocytes from patients with chronic granulocytic leukemia tend to 
have somewhat higher glutamic acid and somewhat lower taurine levels than normal 
platelets and leukocytes. 
Figs. 85-87 show the free amino acid pools of three different preparations of 
neutrophilic polymorphonuclear leukocytes isolated from normal human _ blood. 
These preparations are shown at a lower aliquot size (equivalent to 50 mg of packed 
leukocytes). 
Fig. 88 presents the free amino acid pool of leukocytes obtained from a bone 
marrow puncture of patient E. McG. whose peripheral blood leukocyte free amino 
acids are shown in Fig. 83. Note the increased amount of ethanolamine phosphate 
in particular as compared to the peripheral blood cells. The glutamic and aspartic 
acid levels are also higher while the taurine level is reduced in cells from bone marrow. 
Most bone marrow specimens large enough to examine by paper chromatography 
contained so much venous sinus blood that the cell populations and chromatographic 
results were similar to those from peripheral blood. 
Fig. 89 shows the free amino acid pool in an almost pure preparation of myelocytes. 
The blood sample was drawn from a patient with a white cell count of 125 000/mm*%. 
The white cell preparations from the patient were 92° or more myelocytes. Only a 
few polymorphonuclear leukocytes and myeloblasts were seen in the peripheral 
blood. Note the higher level of ethanolamine phosphate in particular in the myelo- 
cytes as compared to neutrophilic polymorphonuclear leukocytes from leukemia 
References p. 447/448 
