FREE AMINO ACIDS IN BLOOD. II 381 
PLASMA 97 100 
a 
* 
* 
. 
’ 
a 
RBC 98 101 

WBC 99 102 
ma 
<< * 
te HS 
Figs. 97-102. Illustrate the findings in the blood of normal and leukemic dogs. Figs. 97-99 from 
extracts of 0.3 ml of plasma and erythrocytes, and 50 mg of leukocytes from a normal dog. 
Figs. 100-102 from extracts of 0.3 ml of plasma and erythrocytes and 50 mg of leukocytes from 
a dog with granulocvtic leukemia. The arrows indicate the marked elevation of glutamic acid 
in the plasma, erythrocytes, and ieukocytes and the increase in aspartic acid in plasma and 
leukocytes of the leukemic dog. Note the extremely low level of glutamine in erythrocytes and 
leukocytes fiom the leukemic dog. 

volumes were 1230 ml, 855 ml, and 740 ml on the first, second, and third days, 
respectively. For each paper chromatogram an equivalent of 0.5 ml of urine was 
applied to paper. Aside from the gradual decrease in the content of 1-methylhistidine, 
free amino acids remained surprisingly constant over the three day period. Fig. 108 
References p. 447/448 
