FREE AMINO ACIDS IN BLOOD. IV 407 
the withdrawal of the several 4.0-ml blood samples. There was an initial rise in the 
total leukocyte count followed by a marked drop. The differential counts showed 
clearly that the granulocytes were responsible for the increase. Subsequently the 
granulocytes dropped to very low levels. The lymphocyte counts showed a steady 
decline throughout the period of study. 
Tissue samples removed from 4 animals sacrificed at the end of 4 days are shown 
in Figs. 169-180. Figs. 169-171 were prepared from samples of kidney, spleen, and 
bone marrow, respectively, of a typical control animal (subjected to the same con- 
ditions of study except not given mustard). Corresponding samples from 2 animals 
given 2.5 mg/kg of mustard and 1 animal given 1 mg/kg are shown in Figs. 172-180. 
Animal R-3 (Figs. 172-174) showed the maximum response with a marked fall in 
all free amino acids in all tissues. Animal R-rr (Figs. 175-177) showed the response 
typical of 3 of the 4 animals given 2.5 mg/kg of drug. Taurine was reduced in all tis- 
sues and cysteic acid (from cystine and cysteine) increased strikingly in kidney while 

> 
& WN 
RS 
Hematocrit 
WwW 
° 
LEUKOCYTE COUNT 
(% of contro/) 

o—o Granulocytes 
ommmmo Total Leukocytes 
ems— Lymphocytes 
O--- Hematocrit 
Figs. 183. Hematological response of rabbits after administration of nitrogen mustard. “A” the 
average of 4 animals after 2.5 mg/kg, “B” the average response of 3 animals after 1.0 mg/kg. 
The hematocrit value (A), granulocyte count (light line), total leukocyte count (heavy line), 
and the lymphocyte count (broken line) are plotted. 
glutathione increased in spleen and bone marrow. Glutamate and aspartate increased 
in kidney, spleen, and bone marrow. The increase of glutamate in the marrow of 
animal R-11 (Fig. 177) was not as large as the increases observed for the other 
animals. Animal R-6 (Figs. 178-180) that received 1 mg/kg of mustard showed 
changes similar to those observed for R-11 (Figs. 175-177). 
Liver and appendix were examined but are not illustrated. The changes in free 
amino acids seen in appendix were almost identical to those seen in spleen. The 
changes in the liver were similar to those in kidney except that the changes were 
not as great and taurine rose in liver. 
Figs. 181 and 182 show erythrocyte samples removed from animal R-8 (1 mg/kg of 
mustard). The free amino acid level prior to drug administration is shown in Fig. 181 
and the pattern 5 h after drug in Fig. 182. Glutathione and glutamic acid were in- 
References p. 447/448 
