FREE AMINO ACIDS IN BLOOD. V 417 
Marked effects were observed on leukocyte free amino acids of patient M. Fal. in 
two studies. The findings are described in detail below (see also Figs. 232-249). 
The total free amino acid pool decreased and new compounds appeared. The leukocyte 
count was reduced to a lower level than observed for other patients so that maximum 
free amino acid changes in leukocytes also appear to be associated with maximum 
changes in the leukocyte count. 
The effects of CB 2432 (isomeric dimethylmyleran) 
Patient R. Tap. showed minimal effect on free amino acids of plasma, erythrocytes, 
and leukocytes coincident with a minor effect of the drug on the leukocyte count. 
Patient D. Fol. showed more marked changes in both leukocyte count and blood 
free amino acids. A steady decline of plasma glutamic acid, aspartic acid, and taurine 
levels was observed from the sixth through the twentieth day post treatment. The 
changes were most marked when the white cell count was reduced to the greatest 
extent. A marked overall increase of erythrocyte free amino acids six days after 
drug administration was observed for this patient. The increase in free amino acids 
is in contrast to the effects of dimethylmyleran with which decreases of free amino 
acids were produced. Patient D. Fol. showed some white cell free amino acid changes 
after CB 2432. Six days after receiving drug there was an overall decrease of free 
amino acids. On the thirteenth day after drug administration control levels were 
observed for the first time and samples taken on the 28th, 32nd, 34th, 42nd, 5oth, 
and 62nd days post treatment were similar to pretreatment samples. 
Effects of dimethylmyleran on free amino acids in chronic lymphatic leukemia (patient 
R. Tho.) 
The patient was studied in order to contrast the effects of dimethylmyleran in the 
two types of chronic leukemias. The patient received 40 mg of dimethylmyleran 
intravenously and the levels of free amino acids in blood were followed for 23 days. 
Dimethylmyleran did not change the leukocyte count. 
Figs. 195-204 show the free amino acids of blood plasma of the patient after 
dimethylmyleran administration. Some of the pretreatment samples are presented in 
part VI (Figs. 324-336). Fig. 195 shows the plasma sample obtained 16 h after the 
administration of dimethylmyleran. No distinct change from the pretreatment level 
was observed. Fig. 197 shows that 2 days after administration of dimethylmyleran 
there was a fall in the levels of most of the free amino acids. The greatest reductions 
are seen in the aspartate and glutamate levels. A rapid return toward pretreatment 
levels was observed (Figs. 198, 199 on the 4th and 6th days) and was complete in 
8 days (Fig. 200). Throughout the remainder of the study (Figs. 211-214) the plasma 
samples were relatively constant and no effects of the drug were apparent. 
Figs. 205-214 show the chromatograms prepared for erythrocyte samples obtained 
at the same time intervals. A high degree of reproducibility of the erythrocyte pattern 
is clearly indicated. No drug induced changes are apparent. The reliability of the 
methods employed is demonstrated and the absence of uncontrolled effects that 
might be confused with drug effects is clear. 
Figs. 215-224 show the findings with leukocyte samples from patient R. Tho. 
There was a distinct elevation of free amino acids in the leukocytes 2-4 days post 
treatment (Figs. 217, 218). The increase was particularly evident for glutamic acid 
References p. 447/448 
