432 G. ROUSER et al. 
glutamine level gradually rose to a high point between 60—go min (Figs. 272 and 273) 
and then fell gradually almost to the control level by 240 min. A rise in the glu- 
tamic acid level was observed along with the rise in glutamine. An uncharacterized 
substance migrating to the lower left of glutamic acid on chromatograms was also 
observed to rise and fall with the glutamine and glutamate levels. Some fluctuation 
of the taurine level is apparent in the plasma samples and this and fluctuations of the 
glutamic acid level are probably related to the venipuncture response described in 
part I. The venipuncture response may also account for a fall in the alanine level 
observed at 30, 60, and go min (Figs. 270-273). 
Chromatograms prepared from the erythrocyte samples from subject E. Rob. are 
shown in Figs. 278-287. A distinct fall and then an increase of glutamine was ob- 
served in erythrocytes. The highest glutamine level was observed at go min (Fig. 283) 
when the plasma level was highest (Fig. 273). The glutamine level fell below the 
control level at 240 min. A general fall in free amino acid levels in erythrocytes was 
evident between 180 and 240 min. Of considerable interest are the variations that 
occurred during the study. There was a steady decrease of taurine in the first three 
samples (Figs. 278-280) with a rise, a fall, and another rise followed by a fall to 
below the control level at 240 min. These fluctuations are probably related to the 
venipuncture response and to the intake of water by the subject during the study. 
The alanine concentration of erythrocytes decreased then increased and finally 
returned to slightly below the control level at 240 min. The maximum level of ala- 
nine was reached at go min when glutamine was highest. Unlike plasma, the glutamic 
acid level of erythrocytes did not increase, and the uncharacterized compound seen in 
plasma was not detectable in red cells. A more marked venipuncture response in this 
subject may be related to the fact that he was the first to be studied and was apprehen- 
sive throughout the study. The subject complained of slight headache and mild nausea 
one hour after ingestion of glutamine but the symptoms passed quickly. 
Typical urine findings are shown in Figs. 288-295. Figs. 288-292 show the free 
amino acids in the urine specimens from subject E. Rob. before ingestion of glutamine 
(Fig. 288) and 1, 3, 5, and 8 h after glutamine ingestion. A diluted urine was excreted 
for a time and then the free amino acid levels were again near the control levels. 
Figs. 293-295 show three samples obtained from subject H. Bie. after glutamine in- 
jection. A control specimen (Fig. 293) was collected 5 min before the control blood 
sample was drawn and then specimens were obtained go and 330 min after glutamine 
ingestion. The second sample was low in free amino acids and the final sample was 
back to nearly control levels. 
The urine chromatograms show that the large amount of glutamine ingested does 
not increase the excretion of glutamine or other free amino acids to any extent. 
Urinary urea and uric acid were not increased after glutamine ingestion. 
Another normal male subject (A. Knu.) ingested 50 g of glutamine in a total of 
500 ml of water over a period of 4 min. One control blood sample was drawn and 
samples were obtained 30, 60, go, 150, 210, 270 and 330 min following ingestion of 
glutamine. The subject was not as apprehensive as subject E. Rob., but like the latter 
subject experienced mild nausea that passed quickly about 1h after ingestion of 
glutamine. The findings in blood plasma were similar to those with subject E. Rob. 
The highest levels of glutamine in plasma were noted between 60 and go min and 
the levels of both glutamic acid and the uncharacterized substance migrating just to 
References p. 447/448 
