FREE AMINO ACIDS FOLLOWING HEPATECTOMY 451 
maintained without insulin received glucose by continuous infusion at the rate of 
10-100 mg/Ioo g of rat/h. 
Rats after evisceration are unable to maintain normal body temperature and their 
temperature gradually approaches within a few degrees of that of the surrounding 
air!4. In order to maintain more nearly normal body temperatures, some of the rats 
were placed in a warm cabinet. When the temperature of the cabinet was kept at 34°, 
the temperature of the eviscerated rats varied from 36.0 to 37.8°. INGLE AND NEZA- 
mis have compared the times of survival of eviscerated rats at temperatures from 
24° to 38°, and found the longest survival at 26°. When the rats at this temperature 
Rat plasma after evisceration 
gPlus adrenalectomy— 



, ae 
male Total free amino acids X=>32° 
| With insulin 
70} | 
x ---- Without insulin 
60+ oe x 
50+ a 
SD oA Ti 
© 40r mee | 
S | 77 ua o 
Seah rie Bice I | Boa ra 
ey | Gm Fh % oa 
Q 20-r oa 7 pe 
= 10+ KEE Sees 
x: = 
eSeXo hee a 
= Glutamine 
8 aol 
is 
Seal | | 



Hours 
Fig. 1. Total free amino acids in plasma of ten normal rats (open circle )and in groups of 11 and 
12 eviscerated rats maintained at less than 32° for 24 or 48h with insulin or for 24 h without 
insulin. The average concentrations of amino acids are also shown for groups of 11 rats maintained 
at a higher temperature than 32° for 6 and 24 h without insulin and for 24 h with insulin. Standard 
errors are indicated by the vertical lines above and below the average values except when the 
standard error is less than the height of the symbol used. Values for glutamime content are those 
for the same groups of rats. From six to nine rats were included in each group that had both 
evisceration and adrenalectomy. 
received penicillin and streptomycin, the average time of survival was extended to 
approximately 100 h. Our studies also show that rats with temperatures of less than 
32° survived much longer than those of more than 32°. 
Injections of ammonium citrate were given to one normal and one eviscerated rat 
at the rate of 1.0 mg of N/h and to another eviscerated rat at the rate of 0.25 mg 
of N/h. Glutamine was injected in normal and eviscerated rats for 3 and 6h at the 
rate of to mg of a-amino N/h and to other rats for longer periods at the rate of 
0.25 or Img of a-amino N/h. A commercial preparation of amino acids for intra- 
venous injection (elamine) was administered to a few rats. 
Determination of total free amino acids and glutamine. Blood, brain (cerebrum), 
skeletal muscle and cardiac muscle (ventricle) were removed 6, 24 and 48h after 
References p. 460 
