456 E. V. FLOCK AND J. L. BOLLMAN 
TABLE II 
EFFECT OF INJECTION OF GLUTAMINE AND PROTEIN HYDROLYSATE ON FREE AMINO ACIDS 



mg of a-amino N|r00 g of rat 

Plasma Brain Skeletal Cardiac 






Number h after Injection of muscle muscle 
of eviscer- ay == +. = = —— = 
rats en ee a fi Total Total Total Total 
ine free Gluta- free Gluta- free Gluta- free Gluta- 
we N|h h amino mine amino mine amino mine amino mine 
no acids acids acids acids 
rol 10 3 2.6 O:2 2088 5-7 28-3 Tee 20.0% 7.9 
IN 10 3 oO O22 2952 2.9 24.4 Thee 
118) 5 10 Bee Wap HOME | G7 1) OLS) RO Biles} ABA5® § 12633 
IE 4 10 3 14.9 Terme 315-77 0:3, 42-0) “18:2 
ls 15 16 3 Bysshe eee NCeYO) TP eXoL | Arsh) 395255 2316 
1 2 20 16 3 BYSOy ANAS} SY 7A) AL(S-{0) 
IE DR 10 3 26.2 TO) ADS a L723 23 eee lales (6 39:4" 2nd: 
als: 21 10 3 24.1 9.6 By Zee AR GT, 
LE Gy 10 6 21.9 125 Oe A027) 02 Oxo 2 O53 a2 37.07 eer 
IE 29 10 6 723% Tk 1}3) 3Ye), LOs3) 9 3055) LOLS 
Injection of protein 
hydrolysate 
mg of N/h h 
IE 25 16 6 pansy nize) XSR2 itz 44.5** 19.0 
LE 24 16 6 275 TAOe 1 Oa5h0 320) 53026 9.4 
1 1B 24 16 6 44.4 S55} Zi) digit) SX0)43} WONG) 49.9 17.6 
Tole 42 16 10 142.6 223 A329 555 Olt seLOg IOI.1 26.2 

* Cardiac muscle from two rats was combined for analysis. 
** Glutamine was administered toward the end of the experiment. 
*** Plasma from two rats was combined. 
CHROMATOGRAPHIC STUDIES 
Brain. The large increase in concentration of glutamine described in the brain of 
the eviscerated rat was also conspicuous on the two-dimensional paper chromato- 
grams of the brain (Figs. 5, 6). This increase in glutamine occurred without a de- 
crease in the content of glutamic acid as in the dehepatized dog*. Phenylalanine 
and tyrosine, which were scarcely visible when a 1o0o-mg sample of normal brain 
was chromatographed, were clearly visible on these chromatograms of brains of 
eviscerated rats. The y-aminobutyric acid spot looked larger on many of the chroma- 
tograms of brains from eviscerated rats than on those of normal rats. It would be 
desirable to measure this amino acid by a more quantitative method. 
Skeletal muscle. In addition to the marked increase in glutamine in the muscles of 
eviscerated rats which survived for 24h without insulin, increases were noted in 
phenylalanine, arginine, lysine, tyrosine, alanine, threonine, glycine and serine 
(Fig. 7). The increases in these amino acids were less in eviscerated rats which 
received insulin during this period. Although in the dehepatized dog? the content of 
References p. 460 
