FREE AMINO ACIDS IN BLOOD AND TISSUES 457 
un 
=_ 
21 

| : Ean “ 
Fig. 5*. Chromatograms of the free amino acids of the cerebrum. Left, normal rat. Right, an 
eviscerated rat which had survived 1 day after operation and had been maintained with insulin 
and glucose; the body temperature of the eviscerated rat was less than 32°. Aliquots correspond- 
ing to 100 mg of tissue were applied to each sheet at spot X. The sheets were developed in the 
vertical direction with 75°% solution of phenol in the presence of sodium cyanide and ammonia, 
and in the horizontal direction in 65° solution of lutidine containing diethylamine. 
* Composite key to the numbers are: 1, phenylalanine; 2, leucine; 3, valine; 5, arginine; 6, lysi- 
ne; 7, tyrosine; 8, alanine; 9, threonine; 10, glutamine; 11, taurine; 12, glycine; 13, serine; 14, glu- 
tamic acid; 15, carnosine; 16, aspartic acid; 18, histidine; 19, glutathione; 20, y-aminobutyric 
acid; 21, unknown. 
Cerebrum 

Ros A! 
Fig. 6. Chromatograms of the free amino acids of the brain. Left, normal rat. Right, an eviscerated 
rat which had survived 1 day without insulin; at the end of the experiment in this rat, the rectal 
temperature was 32.9°. Samples of tissues used weighed too mg. (See footnote of Fig. 5 for key 
to the numbers). 
