458 E. V. FLOCK AND J. L. BOLLMAN 
glutamic acid in the muscle decreased as the content of glutamine increased, a definite 
decrease in the glutamic acid in the rat was not apparent. 
Heart. Glutamine and many other amino acids increase after evisceration in the rat 
and particularly in rats without insulin (Fig. 8). 

Muscle uscle « 
15 
2 15 : : 
5 3 
: ¢ 
7 6 » & 
8 
10 
hea * ¥ 
e 
B+ «- 
Veer: 
a 4 
16 16 
19 19 
x 
Fig. 7. Chromatograms of the free amino acids of skeletal muscle. Left, normal rat. Right, same 
eviscerated rat as in Fig. 6. (See footnote of fig. 5 for key to the numbers). 
HEAR] 
al ‘ : 2\ 

Fy 
ret hae a 
Fig. 8. Chromatograms of free amino acids from cardiac muscles of two rats (combined). Left, 
two normal rats. Right, two eviscerated rats maintained for 1 day after operation without insulin. 
Rectal temperatures of these two 1ats were 32.9° at end of study. (See footnote of fig. 5 for key 
to the numbers): 
Refevences p. 460 
