466 H. H. TALLAN 
5.0 ml. A 2-ml aliquot of the HCl fraction was heated for 1 h in a boiling-water bath 
to effect hydrolysis of the acetylaspartic acid. This solution and a second, un- 
heated 2-ml aliquot of the HCl effluent were individually taken to dryness three 
times in a rotary evaporator; the residues were dissolved in citrate buffer of pH 2.2 
and both were analyzed for aspartic acid. The amount of acetylaspartic acid was 
calculated from the increase in aspartic acid after hydrolysis. 
The results of the analyses are presented in Table I. It is clear that, for the most 
part, profound changes do not occur in the concentrations of practically all of the 
amino acids. This is in accord with the studies of OKUMURA et al.?, of WRIGHT ef al.3, 
and of Roperts and his group, who investigated the great majority of the amino 
acids for possible changes in concentration under the influence of various psycho- 
tropic drugs. Nevertheless, it was felt that in spite of the usual heavy emphasis in 
TABLE I 
AMINO ACIDS AND RELATED COMPOUNDS IN RAT BRAIN 
Values are expressed in wmoles/g. N.D., non-detectable. For dosage schedules and treatment of 
animals, see the text. Reserpine Group 1 sacrificed 18h after drug, Group 2 sacrificed at 3h. 
The weights of the animals are given at the head of each column. 




N , “ ess : sh : Hydroxyl- 
Normal Imipramine methyl Chlorpromazine Reserpine nine 
Constituent ae 
mine 
I 2 3 I 2 ig 2 I 2 
I70§ 2308 1508 I538 1538 1438 T55 § 180g 1308 T50§ 1708 
Glycerophosphoetha- 
nolamine O550) 109415, 10:60) Ors 21-04) 910363) 10259) 10200. ete 4) a 0:0 78 OL Or7 
Phosphoethanol- 
amine TE QO Ie 72 62 OB ye TA Ob ee 2200) eal Oil at 2tO2 mec O4 ae oO LOm sn SO mmmleg iG 
Taurine 5.9 Bett 6.9 59) 6.0 6.6 6.4 4.2 5.9 8.1 5.6 
Urea 4.8 3.5 Pasg 353} 4.3 4.8 5.5 5-4 6.8 4.3 3.9 
Aspartic acid 2.75) SIRO 2.725) 9 2:00) BIST 27 502.738" 25 On 1b2-715, 9 2 OO meets 
Threonine 0:60) TOl25) FON57a) Ox4'5) 20555) FOlOl= 10:80), (0157 10-75) FO: Onm Oram 
Serine 6:91 (0:745, (0.90) (0168 1:04) 0:07, 0:97, 0:88) 31-07) 40:89) ows 
Glutamine 5.4 4.9 6.0 3.9 5 5.3 6.2 5. 5.6 (O11 5-3 
Glutathione (total, 
as —SH) 2A TBS, BUSAN 0:08) 62:07" SILOpe 2-20) Talli te2.51) beleO7m eilean 
Proline 0:04) 0,00), race) 0:06) race” Trace! 70.02) 0.04) SN; Ds so:00) Onno 
Glutamic acid r1-3 O)i3) 99 My? Olay) 11S KS} sbite(6) = « AKGs'5} Om *l24 Liege On7) 
Glycine T+ 2OVN (oD O4t Ds 2O7k) LOAM SOMES Se Lee 5 | COSMTIh2 7amely  OMmmnEeeE 
Alanine 17/15 901025 0:04) SOLA) 50:74 0.09) 10100 8 50) 57am On7 Sm On am On7S 
Valine OL07)) OL09))) (0.09) | OF05)) C100) | Onl) Osi) (OL08) 0100 NN 0:05) O:G9 
Cystathionine ©1008 0103) O05 10105)" ©.05 8 O00) 10104)" 0.05) 8 0100) OLOO" 7000 
Methionine ©1044) “0102 970.06) | 70107, 0:05, Viraceolo4) Volos i io: oOmNOs02 O00 
Isoleucine ©103)9 7 01025 10103" {0102) O08) | O;04) 0,047) 10:08 O08 is 0:03 aaron 
Leucine ©1060, 0:07) 10:07) (0:06) | (0}08' 410.07), 10:09))10,07, 10:05 10,00) 0569 
Tyrosine 0.04 0.03 0.04 0.05 0.05 0.05 0.04 0.04 0.06 0.06 0.09 
Phenylalanine OLO2s 0102s 9 OL03) 9 10:04 5 (OLO51 ©1024) BOLO 980105 O04 NO O77) olor! 
AMINO DULYIIG ACG 42409) 5 2-000 2555) 2-3 82-43) 23400 2.4 Olen 5 m2 O ee (Om mes Ol 
Ornithine O102) 0:02.) races O.01 “0:00 "0:02" “G108) 0:002 (0.03) 80.01) O02 
Ethanolamine OFZLe fO!25 Na 1OL22namOr2 0530)" (0:2 Fos2 OM RORLOI IT O-.33) 0521 O26 
Ammonia 175 pel A et ul 2S On a lee Sh, (Ov ils ili 2 gl Onna ee 2:02 PLs28eg2-57, 
Lysine OA Ol2S, Ons Ou tOlG 300.225) JO One On Aileen Ong 2mm ©. 42 Ord Onn 2 
Histidine 0:04) 710102) 20:00) 170.077 4 0:04) “race or07) 0:08) (0:01) MO; OOmsG 10 
Arginine ©. LO} 10:08) (0812, | WOWTO} jO:di4s 10} 027090108) 110303) LO;mIOLOSmmOnrA 
Acetylaspartic acid 6.8 723 6.0 Gal 6.6 5.8 5.8 5.3 7.4 5-7 6.8 


References p. 469/470 
