




490 E. MUSSINI AND F. MARCUCCI 
TABICOe 
EFFECT OF DRUGS ON AMINES IN BRAIN 
Brain 
" mg/kg x 6 days == 
Treatment intraperitoneally Serotonin Noradrenaline 
(ug/g) (ug/g) 
Controls 0.39 0.15 
Phenelzine (W 1544) (II) 5 0.95 0.35 
Tranylcypromine (SKF 385) (IV) 5 1.00 0.29 
Iproniazid (1) 100 T.2 0.33 
JB 516 (III) 5 ia? 0.29 
Reserpine (single dose) 2.5 0.10 0.05 
TABLE VI 
EFFECT OF DRUGS ON FREE AMINO ACIDS BEFORE AND AFTER HYDROLYSIS 

Treatment 
mg/kg x 6 days 
intraperitoneally 
Free amino acids in brain after 
picric acid extraction 


Extract Hydrolyzed acid extract 
(mg/roo g) (mg/too g) 
Controls 192 396 
W 1544 (II) 5 189 421 
SKF 385 (IV) 5 185 430 
Iproniazid (1) 100 192 398 
JB 516 (111) 5 200 400 
Reserpine (single dose) 2.5 198 410 

form was increased only by phenelzine. Alanine and GABA were practically un- 
affected. The results obtained with reserpine are summarized in Table VIII. There 
are practically no changes in the pattern of the amino acids. 
Finally the effect of electroshock (electrodes applied to the ears — 110 V; 0.2 sec) 
is reported in Table IX. There are no changes in the concentration of the total amino 
acid before or after hydrolysis when the animals were killed 10 min, 6h, 24h or 
TABLE VII 
FREE AMINO ACIDS IN RAT BRAIN AFTER MAO-INHIBITOR TREATMENT 
Values are expressed in mg/100 g. E means extract, H stands for hydrolyzed extract. 




Controls SKF 385 JB 516 W 1544 Tproniazid 
Amino acid _ - = ~ 
E H E H E H E H E H 
Taurine 30:25 92527 42.3 2A! 25.0 19.2 20.0 23.0 2253 37.5 
Aspartic acid 38.0 63.6 30.0 92.0 33.0 102.0 222) 93.0 26:2") T10;0 
Threonine 9.0 4.4 5.4 4.5 6.5 6.2 2.0 On 5-7 7.3 
Serine 13.0 8.8 Wal gt 8.3 12.5 Wey 7) 4.0 8.8 9.8 Eee 
Asparagine, 
Glutamine 40.6 39.3 — 53.0 = 23.0 — 30.0 —- 
Glutamic acid LO7E 7 Lily, e320) LO2.Os) LOG. LOM ONNE TOS (0) ma 24 1 Onn eLOO aaa STO) 
Alanine 16,3 5 34.6 15.7 32.6 20.3 2U.9 22.0 29.3 16.8 40.2 
GABA 30.0 28.3 22. 30.6 27.0 27.1 21.0 34.0 28.2 20.5 
References p. 492 

