494 W. TH. NAUTA é¢ al. 
as an adjuvant in therapy with neuroleptics such as reserpine and chlorpromazine 
to counteract the extrapyramidal symptoms, and in the treatment of exogenous, 
(reactive) depressions, this substance and related compounds were included in the 
investigation. 
The methods applied have already been described!’ 18. The results have been 
summarised in Tables I, II and III, which also state the testing conditions. 
TABLE I 
THE INFLUENCE OF SOME PSYCHOTROPIC DRUGS ON THE METABOLISM 
OF RAT BRAIN CORTEX 1n vitvo 



Time 002 GABA level (ug/100 mg) Glu level (ug/100 mg) 
Conditions* (min) inhi- 
bition Medium Tissue Total Medium Tissue Total 
Control oO Tae 14.7 20.1 83.2 70.6 153.8 
60 — 2.8 17.0 19.8 Ais Q1.5 TESe2 
Phenobarbital 60 34 2.5 16.3 18.8 2ileon 90.4 Teta) 
Azetazolamide 60 4 2.8 13.9 16.7 21.5 84.2 105.7 
Orphenadrine 60 60 30.4 6.1 30.5 169.4 30.5 199.9 
Chlorpromazine 60 90 34.7 6.1 40.8 197.9 24.6 222.5 

* The concentration of the drugs was 2 mW. Glucose was used as a substrate (concentration: 
0.02 M). Metabolism took place under 100° O,, with a phosphate buffer containing 1 mM Ca. 
After dissection of the animal we found in the cortex 26.4 wg/1oo mg for GABA and 135.0 yg/ 
too mg for Glu. (ERNSTING ef al.17 and RouKEMA!®.) 
Before metabolism is started part of the GABA and Glu leaks from the tissue to 
the medium. However, after 1 h metabolism with glucose as a substrate, the amino 
acids have been resorbed. Metabolism under air, without Ca in the buffer (Table III), 
produces an overall GABA and Glu content of medium plus tissue which approxi- 
TABLE II 
THE INFLUENCE OF PARKINSON DRUGS ON THE METABOLISM 
OF RAT BRAIN CORTEX 2n vitvo 




i Time % Oz GABA level (ug/100 mg) Glu level (ug/roo mg) 
Conditions* (min) inhi- ; a : 
bition Medium Tissue Total Medium Tissue Total 
Control fe) 11.4 14.7 26.1 83.2 70.6 153.8 
60 .- 2.8 17.0 19.8 PB SG) Q1.5 i Ss2 
Trihexyphenidyl 
(Artane R) 60 5 16.5 10.3 20.8 75.6 59.1 134.7 
Procycline 
(Kemadrin ®) 60 7 16.4 8.8 25e2 Q2.1 59.1 151.2 
Cycrimine 
(Pagitane 8) 60 6 15.2 13.8 29.0 59.0 79.7 138.7 
Benzatropine 
methane sulfonate 
(Cogentin 8) 60 88 20.3 3.0 29.3 149.4 20.9 170.3 
Orphenadrine 
(Disipal 8) 60 60 30.4 6.1 30.5 169.4 30.5 199.9 

* For conditions see Table I. 
References p. 498 
