504 Cc. F. BAXTER AND E. ROBERTS 
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Figs. 15-22. Effect of hydroxylamine upon the concentration of amino acids in other body tissues 
of rat. Injected dose NH,OH-HCI, (adjusted to pH 6): 75 mg/kg body wt. Animals were decapi- 
tated 90 min after injection. Chromatograms represent 25 mg equivalent wet weight of tissue. 
Figs. 15, 16: represent the livers of control and treated rats. Arrow points to f-alanine. The 
identity of this amino acid was verified by the greenish color of the spot shortly after develop- 
ment with ninhydrin spray reagent. Since glyceryl phosphoryl ethanolamine has the same Rp 
as f-alanine in our solvent system, an aliquot of brain extract was hydrolyzed (6 N HCl at 100° 
in sealed tube for 24 h). The hydrolysate, after removal of most of the acid, was again examined 
chromatographically. The retention of the spot in question provided further evidence in favor 
of p-alanine. Figs. 17, 18: kidney. Figs. 19-20: muscle. Figs. 21, 22: spleen. All odd numbered 
chromatograms represent tissues from normal control animals; all even numbered chromato- 
grams illustrate changes in the tissues of treated animals. 
