666 S. ARONOFF 
tion* and, by inference, the indole was also. On this rather weak basis it thus appears 
that the young leaf is able to synthesize the indole moiety of tryptophane, but the 
problem obviously needs further study. 
This matter has been pursued somewhat further by the feeding of |U-'C]|shikimic 
acid to — B and + B sunflower leaves, but as Table X shows, there was no appreci- 
TABLE X 
DISTRIBUTION OF RADIOACTIVITY IN NEUTRAL, ANIONIC 
AND CATIONIC FRACTIONS OF TOBACCO LEAVES FED 
[u-14C|SHIKIMIC ACID 
From PERKINS AND ARONOFF, unpublished results. 



Neutral Cationic Anionic 
State aa — naam 
(counts/min) 
+B 16 500 2 760 174 000 
—B 11 800 4 000 205 000 

able differential metabolism of the — B plants. Our efforts to resolve this problem, 
especially with studies of stem-section metabolism, are continuing. 
ACKNOWLEDGEMENTS 
This work was supported by grants from the National Science Foundation and the 
U.S. Atomic Energy Commission. 
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suid on a 100 0%, nici basis. asec the Senha yield was Soci 10%. antic nies the relatively 
large amounts of acid required chromatography onto three sheets. Each sheet thus had 135/3 = 
45 counts/min. The observed count per eluted chromatogram was 40 counts/min, thus suggesting 
that the anthranilate was uniformly labeled. 
