LIVER AND AMINO ACID LEVELS IN BLOOD 709 

suction 
OXIDATION OF 14C-LABELED AMINO ACIDS* BY THE LIVER 
IN 5-H EXPERIMENTS 
Dose 
(mg) 
Amino acid 
L-Phenylalanine 
DL-|2—!4C}|Tryptophane 
L-Arginine - HCl 150 
L- Threonine 
DL-[2—14C|Methionine 2 
L-| 2—14C] Histidine - HCl 
DL-[6—14C]Lysine - HCl 0.6 4 
L-Valine 
L-Leucine 
L-Isoleucine 
L-Glutamic acid 
L-Glutamine 
[1—14C]Glycine 
L-Aspartic acid 
L-Proline 
TABLE I 
Liver perfusion 
25.8 
- 
“Ss 
a 
* All amino acids uniformly labeled unless otherwise 
** Liver left in supplied with hepatic artery. 
+I OSE) Zhe. 
§ Dose, 17.4 mg, duration, 4h. 
§ Duration, 4 h. 
§ Dose, 5.5 mg. 
+ Kidneys also removed. 
§ 
References p. 721 
Fig. 1. Diagram of the apparatus 
used in the perfusion of the isolat- 
ed rat liver. h, humidifier; gm, 
gaseous manometer; p, pump; f, 
filter; t, thermometer; fa, fan; get, 
, Inflow 
cannula; wg, watch glass; oc, out- 
flow cannula; Ip, liver platform; br, 
blood reservoir; ot, overflow tube; 
f-sv, filter side view; grt, gaseous 
return tube; cdt, CO, trap; hc, heat- 
er control; ci, constant infusion. 
From GREEN AND MILLER’. 
gaseous exchange tube; ic 
AND BY NON-HEPATIC TISSUES 
4CO, as per cent dose 
Eviscerated rat 
Liver out Liver in** 
0.13 27.7 
1.0 PEN SP 
3.0% ** 
a2 
2.0 22.0 
0.35 
ek 
19.3 31 
13-3 14.3 
27-588 17-7 
40.2 62.588§ 
20.3 
5.4 19.0 
537 63 
U7 
indicated. 
