710 L. cL. MILLER 
General procedures and details of the chemical methods used in the studies des- 
cribed here have been given in previous publications?. 
Paper-chromatographic studies have been carried out using essentially the tech- 
nique of DENT, phenol saturated with water, and butanol-acetic acid—water (4 : I : 5), 
being the two solvent systems used in two-dimensional chromatographic studies. 
The technique of evisceration used is that of INGLE® and entails removal of all 
abdominal viscera excepting kidneys. In a number of instances the liver was left 
functioning with the intact hepatic artery supplying blood. 
RESULTS 
Part 1. Oxidation of labeled amino acids to 4CO, by the isolated perfused rat liver 
compared with the non-hepatic tissues as exemplified by the eviscerated surviving rat 
Table I lists first a group of amino acids which are oxidized extensively by the 
isolated perfused liver and to a small or insignificant extent by the non-hepatic 
2 
25 S 
20 ISOLATED Seuss 20 SOLATED 
ERFUSED 
% Dose '4c CVeR | Ome %oose '*c terse? O—O 
AS COp '|5 S COo 15 
NON-HEPATIC NON- HEPATIC 
PER HOUR TISSUES @ @ TISSUES 
10 10 —* 
5 5 
ie) ie) 
I 2 3 4 5 | 2 3 4 5 
HOURS HOURS 
Fig. 2 Fig. 3 
Figs. 2-3. Oxidation of L-[!4C]|phenylalanine by the liver compared with non-hepatic tissues. 18-h 
fasted Sprague-Dawley male (weight, 261 g), used as liver donor for perfusion (RLP 371); 
blood volume, 202 ml, to which was added at the outset 14 mg of L-[U-'C]phenylalanine (5 wC) 
and 500 mg of glucose. Duration of perfusion, 6 h. Eviscerated rat (EVR-PA-6), Sprague-Dawley 
male (weight, 212 g), eviscerated under ether anesthesia. 24 mg t-[U-“C]phenylalanine in 3 ml 
of Ringer’s solution injected intravenously at zero time. Duration of experiment, 5 h. 
LIVER IN o—O 
% DOSE !4¢ e a ed a CUMULATIVE 
AS CO, LIVER OUT @—@ % DOSE !4c !5 LIVER OUT @—@ 
PER HOUR AS CO 
10 10 
5 5 
) ) 
le erase aes ioe seas 
HOURS HOURS 
Fig. 4 Fig. 5 
Figs. 4-5. Oxidation of pL-[2-14|Cmethionine in the eviscerated rat. 
Eviscerated surviving rat with liver left functioning supplied by the hepatic artery (EVR-ME-4), 
normal Sprague-Dawley male (weight, 259 g), received a total of 1.3 mg DL-[2-!4C]methionine 
plus 9.6 mg of 1-methionine in approx. 2 ml of Ringer intravenously at the outset of the ex- 
periment. Duration of experiment, 5 h. Eviscerated surviving rat, with liver out, (EVR-ME-2) 
received the same dose of methionine intravenously as above, Duration of experiment, 5 h. 
References p. 721 
