EL. MIP 
N 
HH 
Co 
concentration. These observations are grossly in agreement with changes noted by 
FLock et al.2° and FREEMAN AND SVEC?! incidental to their studies in hepatectomized 
dogs. 
Some of the changes noted in the comparison of initial and final paper chromato- 
grams from isolated liver perfusions have been more firmly documented by quanti- 
tative amino acid estimation made by the procedure of MoorE, SPACKMAN AND 
STEIN*. The following significant observations have been made on initial and final 
plasma specimens from a perfusion in which only glutamine was added at the outset 
of the experiment: (a) As should be apparent from Table II, the gross observations 
made in paper chromatograms concerning the increase in leucine-isoleucine and 
valine are quantitatively most prominent in the column-chromatographic analysis. 
In each case, the relative increase in concentration at the end of 6h perfusion was 
approx. 5-fold. (b) The major decreases in free amino acid concentration were 

361-5 
ty 
+ | % 
361-0 
Fig. 16. Rat liver perfusion (RLP 361). Liver donor, 18-h fasted male Sprague-Dawley (weight, 
g); liver weight, 8. o g. Dose of 43.5 mg L-[U-"] glutamic acid (5 wC). L-glutamic acid and 500 
mg glucose added to 200 ml of blood at start of perfusion. 
* We are indebted to Dr. E. S. Nasser for carrying out these analyses. 
References p. 721 
